SecReT6 ID	791
Strain	Edwardsiella tarda PPD130/91
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	AY424360
Location	3625..24399
Comment	Virulence in blue gourami fish; virulence in several other fish models; invasion of cultured carp epithelial cells
Function	anti-eukaryotic; effector translocation;virulence
T6SS Type	Type i; subtype i4b
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	158512111	90..788 [-], 699	158512111	putative transporting ATPase
2	158512112	739..1581 [-], 843	158512112	hypothetical protein
3	158512113	1654..1821 [-], 168	158512113	hypothetical protein
4	158512114	1843..3297 [-], 1455	158512114	hypothetical protein
5	EvpP (evpP)	3625..4182 [+], 558	158512115	EvpP	EvpP (e)
6	EvpA (evpA)	4431..4946 [+], 516	40287636	EvpA	TssB
7	EvpB (evpB)	4943..6430 [+], 1488	40287637	EvpB	TssC
8	EvpC (evpC)	6500..6991 [+], 492	40287638	EvpC	TssD
9	EvpD (evpD)	7088..8311 [+], 1224	40287639	EvpD
10	EvpE (evpE)	8320..8796 [+], 477	45332447	EvpE	TssE
11	EvpF (evpF)	8800..10641 [+], 1842	158512125	EvpF	TssF
12	EvpG (evpG)	10638..11663 [+], 1026	158512126	EvpG	TssG
13	EvpH (evpH)	11782..14394 [+], 2613	158512127	EvpH	TssH
14	EvpI (evpI)	14394..16379 [+], 1986	158512116	EvpI	TssI
15	EvpJ (evpJ)	16475..16777 [+], 303	158512117	EvpJ	PAAR
16	EvpK (evpK)	16791..17858 [+], 1068	158512118	EvpK	TssA
17	EvpL (evpL)	17779..18486 [+], 708	158512119	EvpL	TssJ
18	EvpM (evpM)	18585..19973 [+], 1389	158512120	EvpM	TssK
19	EvpN (evpN)	19970..20620 [+], 651	158512121	EvpN	TssL
20	EvpO (evpO)	20608..24399 [+], 3792	158512122	EvpO	TssM
21	158512123	24531..25418 [-], 888	158512123	hypothetical protein
22	158512124	25375..26460 [-], 1086	158512124	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files

Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

EvpC , EvpP, EvpI

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	EvpC(evpC)	6500..6991 [+], 492	40287638	EvpC	EvpC
2	EvpP(evpP)	3625..4182 [+], 558	158512115	EvpP	EvpP
3	EvpI(evpI)	14394..16379 [+], 1986	158512116	EvpI	EvpI

Download FASTA format files

Proteins        Genes

Homology immunity protein (immunity protein label, when available)

Regulatory element identified

PhoB-PhoR

Regulator	Description	Reference
PhoB	Activator	PMID: 21953460

Fur

Regulator	Description	Reference
Fur	Repressor	PMID: 21953460

EsrC

Regulator	Description	Reference
EsrC	Activator	PMID: 20937832

PhoP-PhoQ

Regulator	Description	Reference
PhoP	Activator	PMID: 20937832
PhoQ	Activator	PMID: 20937832

EsrB

Regulator	Description	Reference
EsrB	Activator	PMID: 20937832

The information on regulator interaction.

#	homolog effector locus tag	Regulator way	Function	Regulate target	Description
1	VCV52_1108 (TfoX)		+	unknown	.

(1) Hu W et al. (2014). A Disordered Region in the EvpP Protein from the Type VI Secretion System of Edwardsiella tarda is Essential for EvpC Binding. PLoS One. 9(11):e110810. [PudMed:25401506]

(2) Chakraborty S et al. (2011). Two-component PhoB-PhoR regulatory system and ferric uptake regulator sense phosphate and iron to control virulence genes in type III and VI secretion systems of Edwardsiella tarda. J Biol Chem. 286(45):39417-30. [PudMed:21953460]

(3) Chakraborty S et al. (2010). Temperature and Mg2+ sensing by a novel PhoP-PhoQ two-component system for regulation of virulence in Edwardsiella tarda. J Biol Chem. 285(50):38876-88. [PudMed:20937832]

(4) Jobichen C et al. (2010). Structural basis for the secretion of EvpC: a key type VI secretion system protein from Edwardsiella tarda. PLoS One. 5(9):e12910. [PudMed:20886112]

(5) Zheng J et al. (2007). Dissection of a type VI secretion system in Edwardsiella tarda. Mol Microbiol. 66(5):1192-206. [PudMed:17986187]

This literature contains experimental investigation