SecReT6
SecReT6 ID791 experimental
StrainEdwardsiella tarda PPD130/91
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionAY424360
Location3625..24399
CommentVirulence in blue gourami fish; virulence in several other fish models; invasion of cultured carp epithelial cells
Functionanti-eukaryotic; effector translocation;virulence
T6SS TypeType i; subtype i4b
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number111111111
ComponentTssGTssJTssKTssM
Number1111
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1-90..788 [-], 699158512111putative transporting ATPase 
2-739..1581 [-], 843158512112hypothetical protein 
3-1654..1821 [-], 168158512113hypothetical protein 
4-1843..3297 [-], 1455158512114hypothetical protein 
5- (evpP)3625..4182 [+], 558158512115EvpP  EvpP (e)
6- (evpA)4431..4946 [+], 51640287636EvpA  TssB
7- (evpB)4943..6430 [+], 148840287637EvpB  TssC
8- (evpC)6500..6991 [+], 49240287638EvpC  TssD
9- (evpD)7088..8311 [+], 122440287639EvpD 
10- (evpE)8320..8796 [+], 47745332447EvpE  TssE
11- (evpF)8800..10641 [+], 1842158512125EvpF  TssF
12- (evpG)10638..11663 [+], 1026158512126EvpG  TssG
13- (evpH)11782..14394 [+], 2613158512127EvpH  TssH
14- (evpI)14394..16379 [+], 1986158512116EvpI  TssI
15- (evpJ)16475..16777 [+], 303158512117EvpJ  PAAR
16- (evpK)16791..17858 [+], 1068158512118EvpK  TssA
17- (evpL)17779..18486 [+], 708158512119EvpL  TssJ
18- (evpM)18585..19973 [+], 1389158512120EvpM  TssK
19- (evpN)19970..20620 [+], 651158512121EvpN  TssL
20- (evpO)20608..24399 [+], 3792158512122EvpO  TssM
21-24531..25418 [-], 888158512123hypothetical protein 
22-25375..26460 [-], 1086158512124hypothetical protein 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
EvpC , EvpP, EvpI

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1-(evpC)6500..6991 [+], 49240287638EvpC EvpC
2-(evpP)3625..4182 [+], 558158512115EvpP EvpP
3-(evpI)14394..16379 [+], 1986158512116EvpI EvpI

Download FASTA format files
Proteins        Genes
Homology immunity protein (immunity protein label, when available)
Regulatory element identified
PhoB-PhoR
RegulatorDescriptionReference
PhoBActivatorPMID: 21953460

Fur
RegulatorDescriptionReference
FurRepressorPMID: 21953460

EsrC
RegulatorDescriptionReference
EsrCActivatorPMID: 20937832

PhoP-PhoQ
RegulatorDescriptionReference
PhoPActivatorPMID: 20937832
PhoQActivatorPMID: 20937832

EsrB
RegulatorDescriptionReference
EsrBActivatorPMID: 20937832

The information on regulator interaction.

#homolog effector locus tagRegulator wayFunctionRegulate targetDescription
1VCV52_1108 (TfoX)+unknown.
(1) Hu W et al. (2014). A Disordered Region in the EvpP Protein from the Type VI Secretion System of Edwardsiella tarda is Essential for EvpC Binding. PLoS One. 9(11):e110810. [PudMed:25401506] experimental
(2) Chakraborty S et al. (2011). Two-component PhoB-PhoR regulatory system and ferric uptake regulator sense phosphate and iron to control virulence genes in type III and VI secretion systems of Edwardsiella tarda. J Biol Chem. 286(45):39417-30. [PudMed:21953460]
(3) Chakraborty S et al. (2010). Temperature and Mg2+ sensing by a novel PhoP-PhoQ two-component system for regulation of virulence in Edwardsiella tarda. J Biol Chem. 285(50):38876-88. [PudMed:20937832] experimental
(4) Jobichen C et al. (2010). Structural basis for the secretion of EvpC: a key type VI secretion system protein from Edwardsiella tarda. PLoS One. 5(9):e12910. [PudMed:20886112]
(5) Zheng J et al. (2007). Dissection of a type VI secretion system in Edwardsiella tarda. Mol Microbiol. 66(5):1192-206. [PudMed:17986187] experimental
 
experimental This literature contains experimental investigation