SecReT6 ID793 experimental
StrainVibrio alginolyticus EPJS
Repliconchromosome [Browse all T6SS(s) in this replicon]
CommentIn-frame deletion of VEPGS_0008 resulted in obvious biofilm reduction and the complemented strain restored to the level of the wild-type strain. Besides, in-frame deletion of dotU1, VEPGS_0008 and ppkA2 abolished the swarming ability.
Functioneffector translocation
T6SS TypeType i; subtype i1
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1VEPGS_000175..1178 [-], 1104225382144hypothetical protein 
2VEPGS_00021679..2470 [-], 792225382145Dotu family type VI secretion protein  TssL
3VEPGS_00032467..3792 [-], 1326225382146type VI secretion protein  TssK
4VEPGS_00044297..5571 [-], 1275225382147type VI secretion system FHA-containing domain protein  Fha
5VEPGS_00055800..6447 [-], 648225382148hypothetical protein 
6VEPGS_00066951..8264 [+], 1314225382149hypothetical protein  TssA
7VEPGS_00078292..11681 [+], 3390225382150type VI secretion system IcmF  TssM
8VEPGS_000811713..12165 [+], 453225382151transcriptional regulator 
9VEPGS_000912220..12741 [-], 522225382152hypothetical protein  TssG
10VEPGS_001013544..15079 [-], 1536225382153hypothetical protein  TssF
11VEPGS_001115090..15500 [-], 411225382154type VI secretion system lysozyme-related protein  TssE
12VEPGS_001215562..17037 [-], 1476225382155hypothetical protein  TssC
13VEPGS_001317046..17552 [-], 507225382156hypothetical protein  TssB
14VEPGS_001417579..19243 [-], 1665225382157type VI secretion system-associated VCA0119  TssA
15VEPGS_001519230..21230 [-], 2001225382158serine/threonine protein kinase 
16VEPGS_001621468..22412 [-], 945225382159hypothetical protein 
17VEPGS_001722405..22650 [-], 246225382160hypothetical protein 
18VEPGS_001823377..24696 [-], 1320225382161hypothetical protein 
19VEPGS_001924791..25189 [-], 399225382162hypothetical protein 
20VEPGS_002025276..27354 [-], 2079225382163putative VgrG protein  TssI
21VEPGS_002127427..27945 [-], 519225382164uncharacterized hemolysin-coregulated protein  TssD
22VEPGS_002228396..31080 [+], 2685225382165ATPases with chaperone activity ATP-binding subunit  TssH
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
   Hcp1 (VEPGS_0021)
Homology effector (effector protein label, when available)
Homology immunity protein (immunity protein label, when available)
Regulatory element identified
PppA (VEPGS_0613)RepressorPMID: 23021863

LuxOActivatorPMID: 22173829

LuxRCan be activitor or repressorPMID: 22173829

VasHActivatorPMID: 22173829

RpoNActivatorPMID: 22173829

(1) Sheng L et al. (2012). Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 162(2-4):652-62. [PudMed:23021863] experimental
(2) Liu H et al. (2012). Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 55(1):73-81. [PudMed:22563695] experimental
(3) Sheng L et al. (2012). Quorum sensing and alternative sigma factor RpoN regulate type VI secretion system I (T6SSVA1) in fish pathogen Vibrio alginolyticus. Arch Microbiol. 194(5):379-90. [PudMed:22173829] experimental
experimental This literature contains experimental investigation