SecReT6 ID	793
Strain	Vibrio alginolyticus EPJS
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	FJ790776
Location	1..32212
Comment	In-frame deletion of VEPGS_0008 resulted in obvious biofilm reduction and the complemented strain restored to the level of the wild-type strain. Besides, in-frame deletion of dotU1, VEPGS_0008 and ppkA2 abolished the swarming ability.
Function	effector translocation
T6SS Type	Type i; subtype i1
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	2	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	VEPGS_0001	75..1178 [-], 1104	225382144	hypothetical protein
2	VEPGS_0002	1679..2470 [-], 792	225382145	Dotu family type VI secretion protein	TssL
3	VEPGS_0003	2467..3792 [-], 1326	225382146	type VI secretion protein	TssK
4	VEPGS_0004	4297..5571 [-], 1275	225382147	type VI secretion system FHA-containing domain protein	Fha
5	VEPGS_0005	5800..6447 [-], 648	225382148	hypothetical protein
6	VEPGS_0006	6951..8264 [+], 1314	225382149	hypothetical protein	TssA
7	VEPGS_0007	8292..11681 [+], 3390	225382150	type VI secretion system IcmF	TssM
8	VEPGS_0008	11713..12165 [+], 453	225382151	transcriptional regulator
9	VEPGS_0009	12220..12741 [-], 522	225382152	hypothetical protein	TssG
10	VEPGS_0010	13544..15079 [-], 1536	225382153	hypothetical protein	TssF
11	VEPGS_0011	15090..15500 [-], 411	225382154	type VI secretion system lysozyme-related protein	TssE
12	VEPGS_0012	15562..17037 [-], 1476	225382155	hypothetical protein	TssC
13	VEPGS_0013	17046..17552 [-], 507	225382156	hypothetical protein	TssB
14	VEPGS_0014	17579..19243 [-], 1665	225382157	type VI secretion system-associated VCA0119	TssA
15	VEPGS_0015	19230..21230 [-], 2001	225382158	serine/threonine protein kinase
16	VEPGS_0016	21468..22412 [-], 945	225382159	hypothetical protein
17	VEPGS_0017	22405..22650 [-], 246	225382160	hypothetical protein
18	VEPGS_0018	23377..24696 [-], 1320	225382161	hypothetical protein
19	VEPGS_0019	24791..25189 [-], 399	225382162	hypothetical protein
20	VEPGS_0020	25276..27354 [-], 2079	225382163	putative VgrG protein	TssI
21	VEPGS_0021	27427..27945 [-], 519	225382164	uncharacterized hemolysin-coregulated protein	TssD
22	VEPGS_0022	28396..31080 [+], 2685	225382165	ATPases with chaperone activity ATP-binding subunit	TssH

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files

Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

Hcp1 (VEPGS_0021)

Homology effector (effector protein label, when available)

Homology immunity protein (immunity protein label, when available)

Regulatory element identified

PppA

Regulator	Description	Reference
PppA (VEPGS_0613)	Repressor	PMID: 23021863

LuxO

Regulator	Description	Reference
LuxO	Activator	PMID: 22173829

LuxR

Regulator	Description	Reference
LuxR	Can be activitor or repressor	PMID: 22173829

VasH

Regulator	Description	Reference
VasH	Activator	PMID: 22173829

RpoN

Regulator	Description	Reference
RpoN	Activator	PMID: 22173829

(1) Sheng L et al. (2012). Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 162(2-4):652-62. [PudMed:23021863]

(2) Liu H et al. (2012). Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 55(1):73-81. [PudMed:22563695]

(3) Sheng L et al. (2012). Quorum sensing and alternative sigma factor RpoN regulate type VI secretion system I (T6SSVA1) in fish pathogen Vibrio alginolyticus. Arch Microbiol. 194(5):379-90. [PudMed:22173829]

This literature contains experimental investigation