SecReT6 ID	794
Strain	Vibrio alginolyticus EPJS
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	FJ790777
Location	1..31102
Comment	In-frame deletion of VEPGS_0008 resulted in obvious biofilm reduction and the complemented strain restored to the level of the wild-type strain. Besides, in-frame deletion of dotU1, VEPGS_0008 and ppkA2 abolished the swarming ability.
Function	N/A
T6SS Type	Type i; subtype i5
.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	2	1	1	2	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	VEPGS_0601	192..452 [+], 261	225382167	hypothetical protein
2	VEPGS_0602	482..1288 [+], 807	225382168	putative two-component response regulator
3	VEPGS_0603	1687..2148 [+], 462	225382169	Ser/Thr protein kinase
4	VEPGS_0604	2219..2416 [-], 198	225382170	hypothetical protein
5	VEPGS_0605	2445..2780 [-], 336	225382171	hypothetical protein
6	VEPGS_0606	3227..5377 [-], 2151	225382172	serine/threonine protein kinase
7	VEPGS_0607	5690..6775 [+], 1086	225382173	type VI secretion protein FHA-domain containing protein	Fha
8	VEPGS_0608	6921..7685 [+], 765	225791094	hypothetical protein	Fha
9	VEPGS_0609	8163..9452 [+], 1290	225791095	hypothetical protein	TssK
10	VEPGS_0610	9464..10756 [+], 1293	225382176	hypothetical protein	TssL
11	VEPGS_0611	10773..14291 [+], 3519	225382177	type VI secretion system IcmF-related protein	TssM
12	VEPGS_0612	14273..14968 [+], 696	225382178	hypothetical protein
13	VEPGS_0613	14978..15772 [+], 795	225382179	serine/threonine protein phosphatase
14	VEPGS_0614	15765..16880 [+], 1116	225382180	hypothetical protein	TssA
15	VEPGS_0615	16896..17426 [+], 531	225382181	hypothetical protein	TssB
16	VEPGS_0616	17426..18919 [+], 1494	225382182	hypothetical protein	TssC
17	VEPGS_0617	18984..20483 [+], 1500	225382183	hypothetical protein	TssC
18	VEPGS_0618	20494..21288 [+], 795	225382184	hypothetical protein	TagJ
19	VEPGS_0619	21296..21760 [+], 465	225382185	hypothetical protein	TssE
20	VEPGS_0620	21757..23580 [+], 1824	225382186	hypothetical protein	TssF
21	VEPGS_0621	23577..24245 [+], 669	225382187	hypothetical protein	TssG
22	VEPGS_0622	24552..27119 [+], 2568	225382188	putative ClpA/B-type chaperone	TssH
23	VEPGS_0623	27328..27807 [+], 480	225382189	uncharacterized hemolysin-coregulated protein	TssD
24	VEPGS_0624	27857..28789 [+], 933	225382190	Rhs element Vgr protein	TssI
25	VEPGS_0625	28759..29784 [+], 1026	225791096	hypothetical protein	TssI
26	VEPGS_0626	30087..30926 [+], 840	225382192	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files

Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology effector (effector protein label, when available)

Homology immunity protein (immunity protein label, when available)

Regulatory element identified

PppA

Regulator	Description	Reference
PppA (VEPGS_0613)	Activator	PMID: 23021863

The information on regulator interaction.

#	homolog effector locus tag	Regulator way	Function	Regulate target	Description
1	VEPGS_0614 (PppA)	TPP	+	unknown	Phosphorylation of PppA modulates the expression of Hcp2 by regulating LuxR.
2	VEPGS_0606 (PpkA2)	TPP	+	unknown	PppA could be directly or indirectly phosphorylated by PpkA2 at T253 and Phosphorylation of PppA modulates the expression of Hcp2 by regulating LuxR.
3	VEPGS_0606 (PpkA2)	TPP	+	unknown	T6SS2 secretion and killing is controlled by PpkA2 and PpkA2 autophosphorylation at residue Thr 59 as well as PpkA2 phosphorylated DotU2 at two threonine residues, T4 and T28 is required for T6SS2 function.
4	VEPGS_0606 (PpkA2)	TPP	+	unknown	PppA could be directly or indirectly phosphorylated by PpkA2 at T253 and Phosphorylation of PppA modulates the expression of Hcp2 by regulating LuxR.
5	VEPGS_0606 (PpkA2)	TPP	+	unknown	T6SS2 secretion and killing is controlled by PpkA2 and PpkA2 autophosphorylation at residue Thr 59 as well as PpkA2 phosphorylated DotU2 at two threonine residues, T4 and T28 is required for T6SS2 function.
6	VEPGS_0607 (Fha2)	TPP	+	VEPGS_0610 (DotU2)	Fha2 interacts DotU and is critical to T6SS activation.

(1) Sheng L et al. (2012). Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 162(2-4):652-62. [PudMed:23021863]

(2) Liu H et al. (2012). Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 55(1):73-81. [PudMed:22563695]

This literature contains experimental investigation