I. Summary

SecReT6 ID	80
Strain	Francisella tularensis subsp. tularensis SCHU S4
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_006570
Location	1773260..1798375
Comment
Function	anti-eukaryotic; effector translocation;virulence
T6SS Type	Type ii (Type FPI)
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	PdpA	PdpB	IglE	VgrG	IglF	IglG	IglH	DotU	IglI
Number	1	1	1	1	1	1	1	1	1
Component	IglJ	PdpC	PdpE	IglD	IglC	IglB	IglA	PdpD
Number	1	1	1	1	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	FTT_1699 (pdpA2)	1773260..1775722 [+], 2463	56708708	hypothetical protein	PdpA
2	FTT_1700 (pdpB2)	1775727..1779008 [+], 3282	56708709	hypothetical protein	PdpB
3	FTT_1701	1778998..1779375 [+], 378	56708710	hypothetical protein	IglE
4	FTT_1702	1779388..1779882 [+], 495	56708711	hypothetical protein	VgrG
5	FTT_1703	1779887..1781617 [+], 1731	56708712	hypothetical protein	IglF
6	FTT_1704	1781618..1782139 [+], 522	56708713	hypothetical protein	IglG
7	FTT_1705	1782152..1783582 [+], 1431	56708714	hypothetical protein	IglH
8	FTT_1706	1783595..1784218 [+], 624	56708715	hypothetical protein	DotU
9	FTT_1707	1784240..1785391 [+], 1152	56708716	hypothetical protein	IglI
10	FTT_1708	1785357..1786130 [+], 774	56708717	hypothetical protein	IglJ
11	FTT_1709 (pdpC2)	1786170..1790156 [+], 3987	56708718	hypothetical protein	PdpC
12	FTT_1710	1790174..1790749 [+], 576	56708719	hypothetical protein	PdpE
13	FTT_1711c (iglD2)	1790756..1791961 [-], 1206	56708720	intracellular growth locus, subunit D	IglD
14	FTT_1712c (iglC2)	1791973..1792608 [-], 636	56708721	intracellular growth locus, subunit C	IglC
15	FTT_1713c (iglB2)	1792619..1794163 [-], 1545	56708722	intracellular growth locus, subunit B	IglB
16	FTT_1714c (iglA2)	1794153..1794743 [-], 591	56708723	intracellular growth locus, subunit A	IglA
17	FTT_1715c (pdpD2)	1794788..1798375 [-], 3588	56708724	hypothetical protein	PdpD
18	FTT_1718c (isftu1)	1800731..1801515 [-], 785	56708742	transposase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

FTT_1347 (VgrG) , FTL_0118 (IglI)

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	FTT_1702	1779388..1779882 [+], 495	56708711	hypothetical protein (VgrG)	FTT_1347 (VgrG)
2	FTT_1707	1784240..1785391 [+], 1152	56708716	hypothetical protein (IglI)	FTL_0118 (IglI)
Data from experimental literature

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Proteins        Genes

N/A

Regulatory element identified

PmrA

Regulator	Description	Reference
PmrA (FTT_1557c)	Activator	PMID: 17452468

MglA

Regulator	Description	Reference
MglA (FTT_1275)	Activator	PMID: 17452468

(1) Long ME et al. (2013). Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice, and reveals a unique phenotype for pdpC. Infect Immun. 81(3):850-61. [PudMed:23275090]

(2) Broms JE et al. (2012). Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631]

(3) Nano FE et al. (2007). The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]

This literature contains experimental investigation