SecReT6
I. Summary
SecReT6 ID80 experimental
StrainFrancisella tularensis subsp. tularensis SCHU S4
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_006570
Location1773260..1798375
Comment
Functionanti-eukaryotic; effector translocation;virulence
T6SS TypeType ii (Type FPI)
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentPdpAPdpBIglEVgrGIglFIglGIglHDotUIglI
Number111111111
ComponentIglJPdpCPdpEIglDIglCIglBIglAPdpD
Number11111111
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1FTT_1699 (pdpA2)1773260..1775722 [+], 246356708708hypothetical protein  PdpA
2FTT_1700 (pdpB2)1775727..1779008 [+], 328256708709hypothetical protein  PdpB
3FTT_17011778998..1779375 [+], 37856708710hypothetical protein  IglE
4FTT_17021779388..1779882 [+], 49556708711hypothetical protein  VgrG
5FTT_17031779887..1781617 [+], 173156708712hypothetical protein  IglF
6FTT_17041781618..1782139 [+], 52256708713hypothetical protein  IglG
7FTT_17051782152..1783582 [+], 143156708714hypothetical protein  IglH
8FTT_17061783595..1784218 [+], 62456708715hypothetical protein  DotU
9FTT_17071784240..1785391 [+], 115256708716hypothetical protein  IglI
10FTT_17081785357..1786130 [+], 77456708717hypothetical protein  IglJ
11FTT_1709 (pdpC2)1786170..1790156 [+], 398756708718hypothetical protein  PdpC
12FTT_17101790174..1790749 [+], 57656708719hypothetical protein  PdpE
13FTT_1711c (iglD2)1790756..1791961 [-], 120656708720intracellular growth locus, subunit D  IglD
14FTT_1712c (iglC2)1791973..1792608 [-], 63656708721intracellular growth locus, subunit C  IglC
15FTT_1713c (iglB2)1792619..1794163 [-], 154556708722intracellular growth locus, subunit B  IglB
16FTT_1714c (iglA2)1794153..1794743 [-], 59156708723intracellular growth locus, subunit A  IglA
17FTT_1715c (pdpD2)1794788..1798375 [-], 358856708724hypothetical protein  PdpD
18FTT_1718c (isftu1)1800731..1801515 [-], 78556708742transposase 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
FTT_1347 (VgrG) , FTL_0118 (IglI)

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1FTT_17021779388..1779882 [+], 49556708711hypothetical protein (VgrG)FTT_1347 (VgrG)
2FTT_17071784240..1785391 [+], 115256708716hypothetical protein (IglI)FTL_0118 (IglI)
Data from experimental literature

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Proteins        Genes
N/A
Regulatory element identified
PmrA
RegulatorDescriptionReference
PmrA (FTT_1557c)ActivatorPMID: 17452468

MglA
RegulatorDescriptionReference
MglA (FTT_1275)ActivatorPMID: 17452468

(1) Long ME et al. (2013). Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice, and reveals a unique phenotype for pdpC. Infect Immun. 81(3):850-61. [PudMed:23275090] experimental
(2) Broms JE et al. (2012). Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631] experimental
(3) Nano FE et al. (2007). The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]
 
experimental This literature contains experimental investigation