I. Summary

SecReT6 ID	807
Strain	Achromobacter xylosoxidans NH44784-1996
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_021285
Location	5474850..5513952
Comment
Function	N/A
T6SS Type	Type i; subtype i4b
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	3	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	NH44784_051271	5470714..5473887 [-], 3174	528986875	COG1526: Uncharacterized protein required for formate dehydrogenase activity
2	NH44784_051281	5473871..5474857 [-], 987	528986876	hypothetical protein
3	NH44784_051291	5474850..5477543 [-], 2694	528986877	VgrG protein	TssI
4	NH44784_051301	5477560..5480244 [-], 2685	528986878	ClpB protein	TssH
5	NH44784_051311	5480264..5481355 [-], 1092	528986879	Uncharacterized protein ImpH/VasB	TssG
6	NH44784_051321	5481328..5483166 [-], 1839	528986880	Protein ImpG/VasA	TssF
7	NH44784_051331	5483220..5483705 [-], 486	528986881	Uncharacterized protein ImpF	TssE
8	NH44784_051341	5483776..5484279 [-], 504	528986882	Uncharacterized protein ImpD	TssD
9	NH44784_051351	5484320..5485810 [-], 1491	528986883	Uncharacterized protein ImpC	TssC
10	NH44784_051361	5485803..5486327 [-], 525	528986884	Uncharacterized protein ImpB	TssB
11	NH44784_051371	5486395..5487027 [-], 633	528986885	FOG: TPR repeat
12	NH44784_051381	5487481..5487846 [+], 366	528986886	hypothetical protein	TssJ
13	NH44784_051391	5487924..5489270 [+], 1347	528986887	Uncharacterized protein ImpJ/VasE	TssK
14	NH44784_051401	5489329..5490108 [+], 780	528986888	Probable transmembrane protein	TssL
15	NH44784_051411	5490134..5493289 [+], 3156	528986889	VgrG protein	TssI
16	NH44784_051421	5493265..5495328 [+], 2064	528986890	hypothetical protein
17	NH44784_051431	5495309..5495950 [+], 642	528986891	hypothetical protein
18	NH44784_051441	5495963..5496658 [-], 696	528986892	hypothetical protein
19	NH44784_051451	5496748..5499048 [-], 2301	528986893	FIG00464863: hypothetical protein
20	NH44784_051461	5499041..5499808 [-], 768	528986894	hypothetical protein
21	NH44784_051471	5499813..5500790 [-], 978	528986895	hypothetical protein
22	NH44784_051481	5500801..5503677 [-], 2877	528986896	VgrG protein	TssI
23	NH44784_051491	5503704..5504630 [-], 927	528986897	Tail fiber protein
24	NH44784_051501	5504948..5505673 [+], 726	528986898	Probable transmembrane protein
25	NH44784_051511	5505744..5506268 [+], 525	528986899	PROBABLE TRANSMEMBRANE PROTEIN
26	NH44784_051521	5506256..5507143 [+], 888	528986900	Hypothetical Protein
27	NH44784_051531	5507167..5510973 [+], 3807	528986901	IcmF-related protein	TssM
28	NH44784_051541	5510970..5511953 [+], 984	528986902	hypothetical protein
29	NH44784_051551	5511956..5512750 [+], 795	528986903	hypothetical protein	TagL
30	NH44784_051561	5512840..5513952 [-], 1113	528986904	Uncharacterized protein ImpA	TssA
31	NH44784_051571	5514128..5514379 [+], 252	528986905	Orf69
32	NH44784_051581	5514416..5515576 [+], 1161	528986906	hypothetical protein	PAAR
33	NH44784_051591	5515663..5515908 [+], 246	528986907	hypothetical protein
34	NH44784_051601	5515909..5516700 [+], 792	528986908	Methionine aminopeptidase
35	NH44784_051611	5516801..5518021 [+], 1221	528986909	MFS permease
36	NH44784_051621	5518195..5518836 [+], 642	528986910	Uncharacterized glutathione S-transferase-like protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

BCAL0343

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	NH44784_051341	5483776..5484279 [-], 504	528986882	Uncharacterized protein ImpD	BCAL0343

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Proteins        Genes

N/A

N/A