I. Summary

SecReT6 ID	81
Strain	Francisella tularensis subsp. tularensis SCHU S4
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_006570
Location	1379925..1405040
Comment
Function	anti-eukaryotic; effector translocation;virulence
T6SS Type	Type ii (Type FPI)
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	PdpA	PdpB	IglE	VgrG	IglF	IglG	IglH	DotU	IglI
Number	1	1	1	1	1	1	1	1	1
Component	IglJ	PdpC	PdpE	IglD	IglC	IglB	IglA	PdpD
Number	1	1	1	1	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	FTT_1344 (pdpA1)	1379925..1382387 [+], 2463	56708400	hypothetical protein	PdpA
2	FTT_1345 (pdpB1)	1382392..1385673 [+], 3282	56708401	hypothetical protein	PdpB
3	FTT_1346	1385663..1386040 [+], 378	56708402	hypothetical protein	IglE
4	FTT_1347	1386053..1386547 [+], 495	56708403	hypothetical protein	VgrG
5	FTT_1348	1386552..1388282 [+], 1731	56708404	hypothetical protein	IglF
6	FTT_1349	1388283..1388804 [+], 522	56708405	hypothetical protein	IglG
7	FTT_1350	1388817..1390247 [+], 1431	56708406	hypothetical protein	IglH
8	FTT_1351	1390260..1390883 [+], 624	56708407	hypothetical protein	DotU
9	FTT_1352	1390905..1392056 [+], 1152	56708408	hypothetical protein	IglI
10	FTT_1353	1392022..1392795 [+], 774	56708409	hypothetical protein	IglJ
11	FTT_1354 (pdpC1)	1392835..1396821 [+], 3987	56708410	hypothetical protein	PdpC
12	FTT_1355	1396839..1397414 [+], 576	56708411	hypothetical protein	PdpE
13	FTT_1356c (iglD1)	1397421..1398626 [-], 1206	56708412	intracellular growth locus, subunit D	IglD
14	FTT_1357c (iglC1)	1398638..1399273 [-], 636	56708413	intracellular growth locus, subunit C	IglC
15	FTT_1358c (iglB1)	1399284..1400828 [-], 1545	56708414	intracellular growth locus, subunit B	IglB
16	FTT_1359c (iglA1)	1400818..1401408 [-], 591	56708415	intracellular growth locus, subunit A	IglA
17	FTT_1360c (pdpD1)	1401453..1405040 [-], 3588	56708416	hypothetical protein	PdpD
18	FTT_1363c (isftu1)	1407396..1408180 [-], 785	56708417	transposase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

FTT_1347 (VgrG) , FTL_0118 (IglI)

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	FTT_1347	1386053..1386547 [+], 495	56708403	hypothetical protein (VgrG)	FTT_1347 (VgrG)
2	FTT_1352	1390905..1392056 [+], 1152	56708408	hypothetical protein (IglI)	FTL_0118 (IglI)
Data from experimental literature

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Proteins        Genes

N/A

Regulatory element identified

PmrA

Regulator	Description	Reference
PmrA (FTT_1557c)	Activator	PMID: 17452468

MglA

Regulator	Description	Reference
MglA (FTT_1275)	Activator	PMID: 17452468

(1) Long ME et al. (2013). Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice, and reveals a unique phenotype for pdpC. Infect Immun. 81(3):850-61. [PudMed:23275090]

(2) Broms JE et al. (2012). Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631]

(3) Nano FE et al. (2007). The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]

This literature contains experimental investigation