I. Summary

SecReT6 ID	812
Strain	Pseudomonas aeruginosa RP73
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_021577
Location	92525..118557
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	2	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	M062_00385	87998..91102 [-], 3105	514412673	serine/threonine protein kinase	PpkA
2	M062_00390	91110..91838 [-], 729	514407704	PppA	PppA
3	M062_00395	91848..92528 [-], 681	514407705	type VI secretion system protein ImpM	TagF
4	M062_00400	92525..96055 [-], 3531	514407706	type VI secretion protein IcmF	TssM
5	M062_00405	96052..97401 [-], 1350	514407707	type VI secretion protein	TssL
6	M062_00410	97408..98742 [-], 1335	514407708	type VI secretion protein	TssK
7	M062_00415	98758..99222 [-], 465	514407709	type VI secretion protein	TssJ
8	M062_00420	99267..100790 [-], 1524	514412674	signal peptide protein	Fha
9	M062_00425	101158..102192 [+], 1035	514407710	type VI secretion protein ImpA	TssA
10	M062_00430	102281..102799 [+], 519	514407711	hypothetical protein	TssB
11	M062_00435	102812..104308 [+], 1497	514407712	EvpB family type VI secretion protein	TssC
12	M062_00440	104384..104872 [+], 489	514407713	hypothetical protein	TssD
13	M062_00445	105040..105885 [+], 846	514407714	virulence protein, SciE type	TagJ
14	M062_00450	105887..106396 [+], 510	514407715	hypothetical protein	TssE
15	M062_00455	106393..108252 [+], 1860	514407716	type VI secretion system protein ImpG	TssF
16	M062_00460	108216..109262 [+], 1047	514407717	type VI secretion protein	TssG
17	M062_00465	109255..111963 [+], 2709	514407718	ATPase AAA	TssH
18	M062_00470	112001..113941 [+], 1941	514407719	type VI secretion protein ImpA	TssI
19	M062_00475	114055..114339 [-], 285	514407720	hypothetical protein	Tsi6 (i)
20	M062_00480	114336..115628 [-], 1293	514412675	hypothetical protein	Tse6 (e)
21	M062_00485	115644..116078 [-], 435	514407721	hypothetical protein
22	M062_00490	116332..118557 [+], 2226	514407722	type VI secretion protein ImpA	TssI
23	M062_00495	118585..119034 [+], 450	514412676	hypothetical protein
24	M062_00500	118964..120163 [+], 1200	514407723	hypothetical protein
25	M062_00505	120160..121197 [+], 1038	514407724	3-oxoacyl-ACP synthase
26	M062_00510	121405..122145 [+], 741	514412677	hypothetical protein
27	M062_00515	122156..123070 [+], 915	514412678	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files

Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PA0085 , PA0091, PA0095, PA1844, PA2684, PA2685, PA2702, PA2774, PA3484, PA0093

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	M062_00440	104384..104872 [+], 489	514407713	hypothetical protein	PA0085
2	M062_00470	112001..113941 [+], 1941	514407719	type VI secretion protein ImpA	PA0091
3	M062_00490	116332..118557 [+], 2226	514407722	type VI secretion protein ImpA	PA0095
4	M062_09645	2036013..2036477 [-], 465	514409252	hypothetical protein	PA1844
5	M062_14250	3107474..3111427 [-], 3954	514409981	hypothetical protein	PA2684
6	M062_14255	3111477..3113546 [-], 2070	514409982	type VI secretion protein ImpA	PA2685
7	M062_14335	3131244..3131720 [+], 477	514409997	hypothetical protein	PA2702
8	M062_14770	3202263..3202850 [-], 588	514410057	hypothetical protein	PA2774
9	M062_18580	3978709..3979935 [+], 1227	514410704	hypothetical protein	PA3484
10	M062_00480	114336..115628 [-], 1293	514412675	hypothetical protein	PA0093

Download FASTA format files

Proteins        Genes

Homology Immunity protein list

PA0092, PA1845, PA2703, PA2775, PA3485, Tsi5

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	M062_00475	114055..114339 [-], 285	514407720	hypothetical protein	PA0092
2	M062_09650	2036519..2037199 [-], 681	514409253	hypothetical protein	PA1845
3	M062_14340	3131730..3131963 [+], 234	514409998	hypothetical protein	PA2703
4	M062_14775	3202855..3203292 [-], 438	514410058	hypothetical protein	PA2775
5	M062_18585	3979932..3980369 [+], 438	514410705	hypothetical protein	PA3485
6	M062_14245	3107286..3107477 [-], 192	514413131	hypothetical protein	Tsi5

Download FASTA format files

Proteins        Genes

N/A