I. Summary

SecReT6 ID	820
Strain	Salmonella enterica subsp. enterica serovar Heidelberg str. 41578
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_021810
Location	2105345..2134054
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	2	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	SEEH1578_10390 (mltD)	2100603..2101970 [-], 1368	525816127	membrane-bound lytic murein transglycosylase D
2	SEEH1578_10395	2102042..2102797 [-], 756	525816128	hydroxyacylglutathione hydrolase
3	SEEH1578_10400	2102832..2103554 [+], 723	525816129	hypothetical protein
4	SEEH1578_10405 (rnhA)	2103551..2104018 [-], 468	525816130	ribonuclease H
5	SEEH1578_10410	2104082..2104813 [+], 732	525816131	DNA polymerase III subunit epsilon
6	SEEH1578_10415	2105147..2105254 [+], 108	525816132	hypothetical protein
7	SEEH1578_10420	2105345..2106400 [-], 1056	525816133	putative cytoplasmic protein	TssA
8	SEEH1578_10425	2106411..2107406 [-], 996	525816134	type VI secretion protein	TssG
9	SEEH1578_10430	2107403..2109286 [-], 1884	525816135	Protein ImpG/VasA	TssF
10	SEEH1578_10435	2109302..2109796 [-], 495	525816136	hypothetical protein	TssE
11	SEEH1578_10440	2109793..2110617 [-], 825	525816137	Protein of avirulence locus ImpE	TagJ
12	SEEH1578_10445	2110604..2111506 [-], 903	525816138	hypothetical protein
13	SEEH1578_10450	2111874..2114513 [+], 2640	525816139	ClpB protein	TssH
14	SEEH1578_10455	2114613..2115155 [+], 543	525816140	hypothetical protein	TssB
15	SEEH1578_10460	2115179..2116687 [+], 1509	525816141	Type VI protein secretion system	TssC
16	SEEH1578_10465	2116740..2117141 [+], 402	525816142	hypothetical protein	STY0300 (e)
17	SEEH1578_10470	2117113..2117538 [+], 426	525816143	hypothetical protein	STY0301 (i)
18	SEEH1578_10475	2117775..2118260 [+], 486	525816144	Protein hcp1	TssD
19	SEEH1578_10480	2118563..2119048 [+], 486	525816145	Type VI secretion system-associated protein	STM0277 (e)
20	SEEH1578_10485	2119045..2119416 [+], 372	525816146	putative periplasmic protein	STM0278 (i)
21	SEEH1578_10490	2119559..2120044 [+], 486	525816147	Protein hcp1	TssD
22	SEEH1578_10495	2120112..2120648 [+], 537	525816148	hypothetical protein	TssJ
23	SEEH1578_10500	2120652..2121995 [+], 1344	525816149	type VI secretion protein	TssK
24	SEEH1578_10505	2121992..2123296 [+], 1305	525816150	hypothetical protein	TssL
25	SEEH1578_10510	2123301..2124074 [+], 774	525816151	hypothetical protein
26	SEEH1578_10515	2124352..2124708 [+], 357	525816152	putative shiga-like toxin A subunit
27	SEEH1578_10520	2124742..2128611 [+], 3870	525816153	Type VI secretion protein IcmF	TssM
28	SEEH1578_10525	2128611..2129378 [+], 768	525816154	type VI secretion system-associated protein
29	SEEH1578_10530	2129421..2129891 [+], 471	525816155	hypothetical protein
30	SEEH1578_10535	2129876..2130337 [+], 462	525816156	hypothetical protein
31	SEEH1578_10540	2130346..2130468 [+], 123	525816157	hypothetical protein
32	SEEH1578_10545	2130465..2130881 [+], 417	525816158	hypothetical protein
33	SEEH1578_10550	2130908..2131426 [+], 519	525816159	SPI-6 associated protein
34	SEEH1578_10555	2131823..2134054 [+], 2232	525816160	VgrG protein	TssI
35	SEEH1578_10560	2134114..2134572 [+], 459	525816161	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

ROD_34191 , STY0300, EvpC, STM0277, EvpC, STY2606

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	SEEH1578_01785	237043..237528 [-], 486	525814414	hypothetical protein	ROD_34191
2	SEEH1578_10465	2116740..2117141 [+], 402	525816142	hypothetical protein	STY0300
3	SEEH1578_10475	2117775..2118260 [+], 486	525816144	Protein hcp1	EvpC
4	SEEH1578_10480	2118563..2119048 [+], 486	525816145	Type VI secretion system-associated protein	STM0277
5	SEEH1578_10490	2119559..2120044 [+], 486	525816147	Protein hcp1	EvpC
6	SEEH1578_21150	4280206..4280574 [+], 369	525818263	NlpC/P60 enzyme	STY2606

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Proteins        Genes

Homology Immunity protein list

STY0301, STM0278, STY2607

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	SEEH1578_10470	2117113..2117538 [+], 426	525816143	hypothetical protein	STY0301
2	SEEH1578_10485	2119045..2119416 [+], 372	525816146	putative periplasmic protein	STM0278
3	SEEH1578_21155	4280574..4281092 [+], 519	525818264	putative lipoprotein	STY2607

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Proteins        Genes

N/A