I. Summary

SecReT6 ID	828
Strain	Salmonella bongori N268-08
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_021870
Location	1415188..1433789
Comment
Function	N/A
T6SS Type	Type i; subtype i4a
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	1

12

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	A464_1420	1410747..1411796 [-], 1050	526227373	Alcohol dehydrogenase
2	A464_1421	1412884..1413843 [-], 960	526227374	hypothetical protein
3	A464_1422	1414016..1414744 [-], 729	526227375	Transcriptional regulator MerR familyassociated with photolyase
4	A464_1423	1414802..1415176 [-], 375	526227376	hypothetical protein
5	A464_1424	1415188..1416111 [-], 924	526227377	Uncharacterized protein ImpA	TssA
6	A464_1425	1416125..1419664 [-], 3540	526227378	IcmF-related protein	TssM
7	A464_1426	1419661..1420407 [-], 747	526227379	Inner membrane protein DotU	TssL
8	A464_1427	1420397..1421683 [-], 1287	526227380	Uncharacterized protein ImpJ/VasE	TssK
9	A464_1428	1421730..1422200 [-], 471	526227381	hypothetical protein
10	A464_1429	1422531..1423082 [+], 552	526227382	Uncharacterized protein ImpB	TssB
11	A464_1430	1423103..1424584 [+], 1482	526227383	Uncharacterized protein ImpC	TssC
12	A464_1431	1424603..1425079 [+], 477	526227384	Putative cytoplasmic protein USSDB7A	TssD
13	A464_1432	1425092..1425469 [+], 378	526227385	putative cytoplasmic protein	TssE
14	A464_1433	1425488..1427209 [+], 1722	526227386	Protein ImpG/VasA	TssF
15	A464_1434	1427173..1428111 [+], 939	526227387	Uncharacterized protein ImpH/VasB	TssG
16	A464_1435	1428102..1430615 [+], 2514	526227388	ClpB protein	TssH
17	A464_1436	1430639..1431655 [+], 1017	526227389	hypothetical protein
18	A464_1437	1431825..1433789 [+], 1965	526227390	VgrG protein	TssI
19	A464_1438	1433789..1434310 [+], 522	526227391	Phophatidylinositol-4-phosphate 5- kinase
20	A464_1439	1434711..1436084 [-], 1374	526227392	Multidrug and toxin extrusion (MATE) familyefflux pump YdhE/NorM
21	A464_1440	1436301..1436942 [+], 642	526227393	Riboflavin synthase eubacterial/eukaryotic
22	A464_1441	1436986..1438134 [-], 1149	526227394	Cyclopropane-fatty-acyl-phospholipid synthase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

ROD_34191 , STY2606

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	A464_1431	1424603..1425079 [+], 477	526227384	Putative cytoplasmic protein USSDB7A	ROD_34191
2	A464_2489	2469996..2470313 [+], 318	526228441	Putative cytoplasmic protein	STY2606

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Proteins        Genes

N/A

N/A