I. Summary

SecReT6 ID	832
Strain	Burkholderia pseudomallei MSHR305
Replicon	chromosome II [Browse all T6SS(s) in this replicon]
Accession	NC_021877
Location	2998229..3056046
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	2	2	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

15

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BDL_5908	2993261..2993713 [-], 453	526456905	acetyltransferase domain protein
2	BDL_5909	2994532..2995092 [+], 561	526458173	binding--dependent transport system inner membrane component family protein
3	BDL_5910	2995079..2995933 [+], 855	526457763	binding--dependent transport system inner membrane component family protein
4	BDL_5911	2995930..2996961 [+], 1032	526457359	bacterial extracellular solute-binding family protein
5	BDL_5912	2998229..3001021 [+], 2793	526459071	Rhs element Vgr family protein	TssI
6	BDL_5913	3001035..3003275 [+], 2241	526457937	hypothetical protein	BTH_I3226 (e)
7	BDL_5914	3003564..3004952 [+], 1389	526459384	hypothetical protein
8	BDL_5915	3004962..3005225 [+], 264	526457626	PAAR motif family protein	PAAR
9	BDL_5916	3005640..3006059 [-], 420	526458752	hypothetical protein
10	BDL_5917	3006079..3006405 [-], 327	526458722	thioredoxin family protein
11	BDL_5918	3006878..3007570 [+], 693	526459385	putative lipoprotein
12	BDL_5919	3008156..3008881 [-], 726	526457342	hypothetical protein
13	BDL_5920 (ctaD)	3008878..3010473 [-], 1596	526458705	cytochrome c oxidase, subunit I
14	BDL_5921	3010878..3011348 [+], 471	526458148	hypothetical protein
15	BDL_5922	3011614..3013080 [-], 1467	526458526	hypothetical protein
16	BDL_5923	3013440..3013904 [+], 465	526458974	hypothetical protein
17	BDL_5924	3015491..3016402 [+], 912	526458125	coiled stalk of trimeric autotransporter adhesin family protein
18	BDL_5925	3016469..3017920 [-], 1452	526457349	phoPQ-activated pathogenicity-related family protein
19	BDL_5926	3018180..3018578 [-], 399	526457037	yfaZ family protein
20	BDL_5927	3019359..3019904 [+], 546	526458764	fimbrial family protein
21	BDL_5928	3019969..3020706 [+], 738	526458744	hypothetical protein
22	BDL_5929	3021047..3023563 [+], 2517	526459203	hypothetical protein
23	BDL_5930	3023556..3024128 [+], 573	526458342	fimbrial family protein
24	BDL_5931	3024160..3024849 [+], 690	526458871	hypothetical protein	TssL
25	BDL_5932	3024852..3026528 [+], 1677	526458508	ompA family protein	TagL
26	BDL_5933	3026918..3027457 [+], 540	526457513	hypothetical protein	TssB
27	BDL_5934	3027491..3028990 [+], 1500	526457389	hypothetical protein	TssC
28	BDL_5935 (hcp1)	3029190..3029672 [+], 483	526457230	protein hcp1	TssD
29	BDL_5936	3029869..3030342 [+], 474	526457553	type VI secretion lipofamily protein	TssJ
30	BDL_5937	3030348..3031697 [+], 1350	526458771	hypothetical protein	TssK
31	BDL_5938	3031694..3032995 [+], 1302	526458912	type VI secretion system OmpA/MotB family protein	TssL
32	BDL_5939	3033010..3036918 [+], 3909	526457684	intracellular multiplication and macrophage-killing family protein	TssM
33	BDL_5940	3037108..3037677 [+], 570	526459129	hypothetical protein
34	BDL_5941	3037775..3040528 [+], 2754	526456866	Rhs element Vgr family protein	TssI
35	BDL_5942	3040536..3040727 [+], 192	526457557	PAAR motif family protein	PAAR
36	BDL_5944	3040740..3044192 [+], 3453	526458632	RHS repeat-associated core domain protein
37	BDL_5943	3044085..3045443 [-], 1359	526457158	glycosyltransferase family protein
38	BDL_5945	3045476..3046504 [-], 1029	526458411	fimbrial family protein
39	BDL_5946	3046559..3047629 [-], 1071	526456964	hypothetical protein	TssA
40	BDL_5947	3047648..3048697 [-], 1050	526457756	hypothetical protein	TssG
41	BDL_5948	3048694..3050574 [-], 1881	526457750	hypothetical protein	TssF
42	BDL_5949	3050576..3051094 [-], 519	526457039	lysozyme family protein	TssE
43	BDL_5950	3051081..3051887 [-], 807	526457043	impE family protein	TagJ
44	BDL_5951	3051919..3052971 [-], 1053	526459283	hypothetical protein
45	BDL_5952 (clpV)	3053401..3056046 [+], 2646	526458755	type VI secretion ATPase, ClpV1 family	TssH
46	BDL_5953	3056437..3059481 [-], 3045	526458420	response regulator

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

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