I. Summary

SecReT6 ID	836
Strain	Rhizobium etli bv. mimosae str. Mim
Replicon	plasmid pRetMIM1f [Browse all T6SS(s) in this replicon]
Accession	NC_021911
Location	491148..515517
Comment
Function	N/A
T6SS Type	Type i; subtype i5
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	2	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	REMIM1_PF00458	486238..486558 [-], 321	528837972	cytochrome-c oxidase subunit 2 protein
2	REMIM1_PF00459	486566..487087 [-], 522	528837973	hypothetical protein
3	REMIM1_PF00460	487187..487534 [-], 348	528837974	hypothetical protein
4	REMIM1_PF00461	487735..488874 [+], 1140	528837975	polysaccharide export protein
5	REMIM1_PF00462	488871..489782 [-], 912	528837976	serine/threonine protein kinase-like domain-containing protein
6	REMIM1_PF00463	489772..491139 [-], 1368	528837977	type VI secretion system-associated protein
7	REMIM1_PF00464	491148..494624 [-], 3477	528837978	type VI secretion system IcmF family protein	TssM
8	REMIM1_PF00465	494624..496162 [-], 1539	528837979	type VI/VI secretion system outer membrane protein	TssL
9	REMIM1_PF00466	496159..497499 [-], 1341	528837980	type VI secretion system-associated protein	TssK
10	REMIM1_PF00467	497489..498673 [-], 1185	528837981	type VI protein secretion system FHA domain-containing protein	Fha
11	REMIM1_PF00468	498684..499685 [-], 1002	528837982	type VI secretion system-associated protein	TssG
12	REMIM1_PF00469	499706..501487 [-], 1782	528837983	type VI secretion system-associated protein	TssF
13	REMIM1_PF00470	501480..501989 [-], 510	528837984	type VI secretion system lysozyme-related protein	TssE
14	REMIM1_PF00471	501982..502812 [-], 831	528837985	type VI secretion system ImpE family protein	TagJ
15	REMIM1_PF00472	502809..504197 [-], 1389	528837986	type VI secretion system-associated protein	TssC
16	REMIM1_PF00473	504268..505749 [-], 1482	528837987	type VI secretion system-associated protein	TssC
17	REMIM1_PF00474	505879..506424 [-], 546	528837988	type VI secretion system-associated protein	TssB
18	REMIM1_PF00475	506486..507550 [-], 1065	528837989	type VI secretion system-associated ImpA-related domain-containing protein	TssA
19	REMIM1_PF00476 (clpV1)	507886..510540 [+], 2655	528837990	type VI secretion system ATPase protein ClpV1	TssH
20	REMIM1_PF00477	510582..511058 [+], 477	528837991	virulence factor secretion protein	TssD
21	REMIM1_PF00478	511204..512286 [+], 1083	528837992	hypothetical protein
22	REMIM1_PF00479	512297..513223 [+], 927	528837993	hypothetical protein
23	REMIM1_PF00480	513253..515517 [+], 2265	528837994	type VI secretion system Rhs element Vgr family protein	TssI
24	REMIM1_PF00481	515531..516640 [+], 1110	528837995	hypothetical protein
25	REMIM1_PF00482	516637..518160 [+], 1524	528837996	PAAR motif-containing protein
26	REMIM1_PF00483	518181..518891 [+], 711	528837997	GAD-like domain-containing protein
27	REMIM1_PF00484	519013..519663 [+], 651	528837998	hypothetical protein
28	REMIM1_PF00485	519729..519944 [+], 216	528837999	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

RbsB , Atu4345

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	REMIM1_CH02415(rbsB-2)	2405399..2406328 [+], 930	528829165	ribose ABC transporter substrate-binding protein RbsB 2	RbsB
2	REMIM1_PF00477	510582..511058 [+], 477	528837991	virulence factor secretion protein	Atu4345

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Proteins        Genes

N/A

N/A