I. Summary

SecReT6 ID	844
Strain	Listonella anguillarum M3
Replicon	chromosome II [Browse all T6SS(s) in this replicon]
Accession	NC_022224
Location	611038..633645
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	0	1	1	2	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

13

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	N175_17320	606121..607818 [-], 1698	537457111	pseudouridine synthase
2	N175_17325	608060..608179 [+], 120	537457463	hypothetical protein
3	N175_17330	608392..609480 [+], 1089	537457112	hypothetical protein
4	N175_17335	609955..610875 [-], 921	537457113	transposase IS903
5	N175_17340	611038..611442 [+], 405	537457114	type VI secretion protein	TssB
6	N175_17345	611489..612967 [+], 1479	537457115	type VI secretion protein	TssC
7	N175_17350	612970..613407 [+], 438	537457116	lysozyme	TssE
8	N175_17355	613414..615183 [+], 1770	537457117	type VI secretion protein	TssF
9	N175_17360	615186..616169 [+], 984	537457464	hypothetical protein	TssG
10	N175_17365	616174..617646 [+], 1473	537457118	signal peptide protein	Fha
11	N175_17370	617649..618128 [+], 480	537457119	type VI secretion protein	TssJ
12	N175_17375	618133..619467 [+], 1335	537457120	hypothetical protein	TssK
13	N175_17380	619467..620243 [+], 777	537457121	type IV / VI secretion system , DotU family domain protein	TssL
14	N175_17385	620258..622885 [+], 2628	537457122	ATPase AAA	TssH
15	N175_17390	622882..624450 [+], 1569	537457123	ATPase AAA	VasH
16	N175_17395	624447..625106 [+], 660	537457124	type VI secretion protein
17	N175_17400	625116..626513 [+], 1398	537457125	type VI secretion protein	TssA
18	N175_17405	626531..630076 [+], 3546	537457126	type VI secretion protein IcmF	TssM
19	N175_17410	630134..631429 [+], 1296	537457127	type VI secretion protein	TssA
20	N175_17415	631561..633645 [+], 2085	537457128	type IV secretion protein Rhs	TssI
21	N175_17420	633621..634442 [+], 822	537457129	hypothetical protein
22	N175_17425	634439..636580 [+], 2142	537457130	VgrG
23	N175_17430	636582..637316 [+], 735	537457131	nitroreductase
24	N175_17435	637339..638082 [+], 744	537457132	nitroreductase
25	N175_17440	638159..638554 [+], 396	537457133	membrane protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

VCA0020 , VCA0018, VCA0017, VCA0017, VC1416, VC1418

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	N175_13420	2841456..2844716 [-], 3261	537455114	hypothetical protein	VCA0020
2	N175_13430	2845600..2847696 [-], 2097	537455116	type IV secretion protein Rhs	VCA0018
3	N175_13435	2847836..2848354 [-], 519	537455117	hypothetical protein	VCA0017
4	N175_16725	471403..471921 [-], 519	537457008	hypothetical protein	VCA0017
5	N175_17415	631561..633645 [+], 2085	537457128	type IV secretion protein Rhs	VC1416
6	N175_18440	887463..889373 [+], 1911	537457295	lipase	VC1418

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Proteins        Genes

Homology Immunity protein list

VCA0021, VC1419

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	N175_13415	2840727..2841455 [-], 729	537455113	hypothetical protein	VCA0021
2	N175_18450	890092..890826 [+], 735	537457297	hypothetical protein	VC1419

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Proteins        Genes

N/A