I. Summary

SecReT6 ID	870
Strain	Pseudomonas aeruginosa PA1R
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_022806
Location	190404..210290
Comment
Function	N/A
T6SS Type	Type i; subtype i4a
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	PA1R_gp0154	185618..186802 [-], 1185	558666116	Acyl-CoA dehydrogenase; probable dibenzothiophene desulfurization enzyme
2	PA1R_gp0155	186832..187977 [-], 1146	558666117	Alkanesulfonate monooxygenase
3	PA1R_gp0156	187987..188547 [-], 561	558666118	FMN reductase
4	PA1R_gp0157	188707..188973 [-], 267	558666119	hypothetical protein
5	PA1R_gp0158	188950..189198 [+], 249	558666120	hypothetical protein
6	PA1R_gp0159	189209..190294 [-], 1086	558666121	Sigma-54 dependent transcriptional regulator	Sfa3
7	PA1R_gp0160	190404..191504 [-], 1101	558666122	Uncharacterized protein ImpA	TssA
8	PA1R_gp0161	191501..195316 [-], 3816	558666123	IcmF-related protein	TssM
9	PA1R_gp0162	195313..196071 [-], 759	558666124	Inner membrane protein DotU	TssL
10	PA1R_gp0163	196089..197381 [-], 1293	558666125	Uncharacterized protein ImpJ/VasE	TssK
11	PA1R_gp0164	197480..197956 [-], 477	558666126	hypothetical protein
12	PA1R_gp0165	198165..198710 [+], 546	558666127	Uncharacterized protein ImpB	TssB
13	PA1R_gp0166	198733..200217 [+], 1485	558666128	Uncharacterized protein ImpC	TssC
14	PA1R_gp0167	200291..200788 [+], 498	558666129	Putative cytoplasmic protein USSDB7A	TssD
15	PA1R_gp0168	200801..201226 [+], 426	558666130	Uncharacterized protein ImpF	TssE
16	PA1R_gp0169	201210..203003 [+], 1794	558666131	Protein ImpG/VasA	TssF
17	PA1R_gp0170	202967..203983 [+], 1017	558666132	Uncharacterized protein ImpH/VasB	TssG
18	PA1R_gp0171	203985..206534 [+], 2550	558666133	ClpB protein	TssH
19	PA1R_gp0172	206608..207141 [+], 534	558666134	hypothetical protein
20	PA1R_gp0173	207249..208247 [+], 999	558666135	hypothetical protein
21	PA1R_gp0174	208284..210290 [+], 2007	558666136	VgrG protein	TssI
22	PA1R_gp0175	210301..210837 [+], 537	558666137	hypothetical protein
23	PA1R_gp0176	210860..211255 [-], 396	558666138	hypothetical protein
24	PA1R_gp0177	211532..212173 [+], 642	558666139	DNA-binding response regulator, LuxR family
25	PA1R_gp0178	212374..213579 [+], 1206	558666140	putative ABC-type transport protein, periplasmic component

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PA5089

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PA1R_gp3016	3373983..3376220 [-], 2238	558668927	hypothetical protein	PA5089

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Proteins        Genes

Homology Immunity protein list

PA5087, PA5087, PA5087, PA5088

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PA1R_gp3012	3370821..3371348 [-], 528	558668923	hypothetical protein	PA5087
2	PA1R_gp3013	3371348..3372226 [-], 879	558668924	hypothetical protein	PA5087
3	PA1R_gp3014	3372226..3373104 [-], 879	558668925	hypothetical protein	PA5087
4	PA1R_gp3015	3373104..3373979 [-], 876	558668926	hypothetical protein	PA5088

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Proteins        Genes

N/A