I. Summary

SecReT6 ID	871
Strain	Pseudomonas aeruginosa PA1R
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_022806
Location	4020263..4046269
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	2	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

14

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	PA1R_gp3588	4015742..4018840 [-], 3099	558669492	Serine/threonine protein kinase	PpkA
2	PA1R_gp3589	4018848..4019576 [-], 729	558669493	Phosphoprotein phosphatase PppA	PppA
3	PA1R_gp3590	4019586..4020266 [-], 681	558669494	Protein phosphatase ImpM	TagF
4	PA1R_gp3591	4020263..4023796 [-], 3534	558669495	IcmF-related protein	TssM
5	PA1R_gp3592	4023793..4025142 [-], 1350	558669496	Outer membrane protein ImpK/VasF, OmpA/MotB domain protein	TssL
6	PA1R_gp3593	4025149..4026483 [-], 1335	558669497	Uncharacterized protein ImpJ/VasE	TssK
7	PA1R_gp3594	4026499..4026963 [-], 465	558669498	Type VI secretion lipoprotein/VasD	TssJ
8	PA1R_gp3595	4027008..4028501 [-], 1494	558669499	Uncharacterized protein ImpI/VasC	Fha
9	PA1R_gp3596	4028869..4029903 [+], 1035	558669500	Uncharacterized protein ImpA	TssA
10	PA1R_gp3597	4029992..4030510 [+], 519	558669501	Uncharacterized protein ImpB	TssB
11	PA1R_gp3598	4030523..4032019 [+], 1497	558669502	Uncharacterized protein ImpC	TssC
12	PA1R_gp3599	4032095..4032583 [+], 489	558669503	Uncharacterized protein ImpD	TssD
13	PA1R_gp3600	4032811..4033596 [+], 786	558669504	Protein of avirulence locus ImpE	TagJ
14	PA1R_gp3601	4033598..4034107 [+], 510	558669505	Uncharacterized protein ImpF	TssE
15	PA1R_gp3602	4034104..4035963 [+], 1860	558669506	Protein ImpG/VasA	TssF
16	PA1R_gp3603	4035927..4036973 [+], 1047	558669507	Uncharacterized protein ImpH/VasB	TssG
17	PA1R_gp3604	4036966..4039674 [+], 2709	558669508	ClpB protein	TssH
18	PA1R_gp3605	4039712..4041652 [+], 1941	558669509	VgrG protein	TssI
19	PA1R_gp3606	4041767..4042051 [-], 285	558669510	hypothetical protein	Tsi6 (i)
20	PA1R_gp3607	4042048..4043340 [-], 1293	558669511	Rhs family protein	Tse6 (e)
21	PA1R_gp3608	4043356..4043790 [-], 435	558669512	hypothetical protein
22	PA1R_gp3609	4044044..4046269 [+], 2226	558669513	VgrG protein	TssI
23	PA1R_gp3610	4046297..4046746 [+], 450	558669514	hypothetical protein
24	PA1R_gp3611	4046763..4047875 [+], 1113	558669515	hypothetical protein
25	PA1R_gp3612	4047872..4048909 [+], 1038	558669516	3-oxoacyl-[ACP] synthase
26	PA1R_gp3613	4048910..4049857 [+], 948	558669517	hypothetical protein
27	PA1R_gp3614	4049868..4050782 [+], 915	558669518	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PA2684 , PA2685, PA0093, PA0091, PA2702, PA2774, PA3484, PA0085, PA0091, PA0093, PA0095, PA1844

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PA1R_gp0488	602419..606372 [-], 3954	558666446	Rhs family protein	PA2684
2	PA1R_gp0489	606422..608491 [-], 2070	558666447	VgrG protein	PA2685
3	PA1R_gp0491	609195..610487 [+], 1293	558666449	Rhs family protein	PA0093
4	PA1R_gp0493	610883..612823 [-], 1941	558666451	VgrG protein	PA0091
5	PA1R_gp0510	631838..632314 [+], 477	558666468	hypothetical protein	PA2702
6	PA1R_gp0595	715106..715693 [-], 588	558666552	hypothetical protein	PA2774
7	PA1R_gp1325	1489399..1490625 [+], 1227	558667263	hypothetical protein	PA3484
8	PA1R_gp3599	4032095..4032583 [+], 489	558669503	Uncharacterized protein ImpD	PA0085
9	PA1R_gp3605	4039712..4041652 [+], 1941	558669509	VgrG protein	PA0091
10	PA1R_gp3607	4042048..4043340 [-], 1293	558669511	Rhs family protein	PA0093
11	PA1R_gp3609	4044044..4046269 [+], 2226	558669513	VgrG protein	PA0095
12	PA1R_gp5470	6069226..6069690 [-], 465	558671355	hypothetical protein	PA1844

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Proteins        Genes

Homology Immunity protein list

PA0092, PA2703, PA2775, PA3485, PA0092, PA1845

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PA1R_gp0492	610484..610768 [+], 285	558666450	hypothetical protein	PA0092
2	PA1R_gp0511	632324..632557 [+], 234	558666469	hypothetical protein	PA2703
3	PA1R_gp0596	715698..716135 [-], 438	558666553	hypothetical protein	PA2775
4	PA1R_gp1326	1490622..1491059 [+], 438	558667264	hypothetical protein	PA3485
5	PA1R_gp3606	4041767..4042051 [-], 285	558669510	hypothetical protein	PA0092
6	PA1R_gp5471	6069732..6070250 [-], 519	558671356	hypothetical protein	PA1845

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Proteins        Genes

N/A