I. Summary

SecReT6 ID	883
Strain	Cronobacter sakazakii CMCC 45402
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_023032
Location	27903..82237
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	3	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	P262_00038	24147..25301 [-], 1155	565643495	multidrug resistance protein D
2	P262_00039	25537..25989 [-], 453	565643496	hypothetical protein
3	P262_00040	26044..26172 [+], 129	565643497	hypothetical protein
4	P262_00041	26590..26724 [-], 135	565643498	hypothetical protein
5	P262_00042	26972..27106 [+], 135	565643499	hypothetical protein
6	P262_00043	27384..27878 [+], 495	565643500	hypothetical protein
7	P262_00044	27903..28409 [+], 507	565643501	hypothetical protein	TssJ
8	P262_00045	28434..29777 [+], 1344	565643502	hypothetical protein	TssK
9	P262_00047	29795..31030 [+], 1236	565643503	hypothetical protein	TssL
10	P262_00048	31032..34649 [+], 3618	565643504	type VI secretion system protein ImpL	TssM
11	P262_00049	34666..35376 [+], 711	565643505	hypothetical protein
12	P262_00051	35386..36408 [+], 1023	565643506	hypothetical protein	TssA
13	P262_00052	36472..37008 [+], 537	565643507	hypothetical protein	TssB
14	P262_00053	37012..38511 [+], 1500	565643508	hypothetical protein	TssC
15	P262_00055	38558..39004 [+], 447	565643509	hypothetical protein
16	P262_00056	39045..39446 [+], 402	565643510	hypothetical protein
17	P262_00058	39652..40134 [+], 483	565643511	hypothetical protein	TssD
18	P262_00061	40182..40550 [+], 369	565643512	hypothetical protein
19	P262_00063	40571..40954 [+], 384	565643513	hypothetical protein
20	P262_00064	41251..43182 [+], 1932	565643514	hypothetical protein	Fha
21	P262_00065	43179..43973 [+], 795	565643515	hypothetical protein
22	P262_00066	43992..44996 [+], 1005	565643516	hypothetical protein
23	P262_00068	45022..45855 [+], 834	565643517	hypothetical protein	TagJ
24	P262_00069	45848..46420 [+], 573	565643518	hypothetical protein	TssE
25	P262_00070	46423..48294 [+], 1872	565643519	hypothetical protein	TssF
26	P262_00071	48291..49334 [+], 1044	565643520	hypothetical protein	TssG
27	P262_00073	49409..49726 [+], 318	565643521	hypothetical protein
28	P262_00077	49773..52388 [+], 2616	565643522	hypothetical protein	TssH
29	P262_00080	52419..53852 [+], 1434	565643523	serine/threonine protein kinase
30	P262_00081	53977..55443 [+], 1467	565643524	hypothetical protein
31	P262_00082	55440..56201 [+], 762	565643525	hypothetical protein
32	P262_00085	56342..58543 [+], 2202	565643526	Rhs element Vgr protein	TssI
33	P262_00088	59227..61767 [+], 2541	565643527	hypothetical protein
34	P262_00090	61764..62834 [+], 1071	565643528	pentapeptide repeat protein
35	P262_00092	62844..63449 [+], 606	565643529	hypothetical protein
36	P262_00093	63455..63847 [+], 393	565643530	hypothetical protein
37	P262_00096	64099..66678 [+], 2580	565643531	hypothetical protein	TssI
38	P262_00097	67167..67370 [+], 204	565643532	hypothetical protein
39	P262_00099	68016..68441 [+], 426	565643533	hypothetical protein
40	P262_00101	68455..72879 [+], 4425	565643534	hypothetical protein	rhsA (e)
41	P262_00103	72867..73451 [+], 585	565643535	hypothetical protein
42	P262_00104	73902..74879 [+], 978	565643536	hypothetical protein
43	P262_00106	75030..76067 [+], 1038	565643537	hypothetical protein
44	P262_00107	76067..77065 [+], 999	565643538	hypothetical protein
45	P262_00108	77291..78064 [+], 774	565643539	hypothetical protein
46	P262_00109	78843..78992 [+], 150	565643540	hypothetical protein
47	P262_00110	79087..79605 [+], 519	565643541	hypothetical protein
48	P262_00111	79589..79849 [-], 261	565643542	hypothetical protein
49	P262_00113	80117..80248 [+], 132	565643543	hypothetical protein
50	P262_00114	80297..82237 [+], 1941	565643544	hypothetical protein	TssI
51	P262_00115	82234..82665 [+], 432	565643545	hypothetical protein
52	P262_00118	82668..87227 [+], 4560	565643546	hypothetical protein	PAAR

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

SMA3455 , SMA2263, ECL_01567

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	P262_p1093	76054..76620 [+], 567	565631949	hypothetical protein	SMA3455
2	P262_00058	39652..40134 [+], 483	565643511	hypothetical protein	SMA2263
3	P262_00101	68455..72879 [+], 4425	565643534	hypothetical protein	ECL_01567

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Proteins        Genes

N/A

N/A