I. Summary

SecReT6 ID	887
Strain	Pseudomonas aeruginosa LES431
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_023066
Location	90607..116604
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	2	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	T223_00385	86086..89184 [-], 3099	568151260	serine/threonine protein kinase	PpkA
2	T223_00390	89192..89920 [-], 729	568151261	PppA	PppA
3	T223_00395	89930..90610 [-], 681	568151262	type VI secretion system protein ImpM	TagF
4	T223_00400	90607..94137 [-], 3531	568151263	type VI secretion protein IcmF	TssM
5	T223_00405	94134..95483 [-], 1350	568151264	type VI secretion protein	TssL
6	T223_00410	95490..96824 [-], 1335	568151265	type VI secretion protein	TssK
7	T223_00415	96840..97304 [-], 465	568151266	type VI secretion protein	TssJ
8	T223_00420	97349..98836 [-], 1488	568151267	signal peptide protein	Fha
9	T223_00425	99204..100238 [+], 1035	568151268	type VI secretion protein ImpA	TssA
10	T223_00430	100327..100845 [+], 519	568151269	hypothetical protein	TssB
11	T223_00435	100858..102354 [+], 1497	568151270	EvpB family type VI secretion protein	TssC
12	T223_00440	102430..102918 [+], 489	568151271	hypothetical protein	TssD
13	T223_00445	103146..103931 [+], 786	568151272	virulence protein, SciE type	TagJ
14	T223_00450	103933..104442 [+], 510	568151273	hypothetical protein	TssE
15	T223_00455	104439..106298 [+], 1860	568151274	type VI secretion system protein ImpG	TssF
16	T223_00460	106262..107308 [+], 1047	568151275	type VI secretion protein	TssG
17	T223_00465	107301..110009 [+], 2709	568151276	ATPase AAA	TssH
18	T223_00470	110047..111987 [+], 1941	568151277	type VI secretion protein ImpA	TssI
19	T223_00475	112102..112386 [-], 285	568151278	hypothetical protein	Tsi6 (i)
20	T223_00490	113691..114125 [-], 435	568151279	hypothetical protein
21	T223_00495	114379..116604 [+], 2226	568151280	type VI secretion protein ImpA	TssI
22	T223_00500	116632..117081 [+], 450	568151281	hypothetical protein
23	T223_00505	117098..118210 [+], 1113	568151282	hypothetical protein
24	T223_00510	118207..119244 [+], 1038	568151283	3-oxoacyl-ACP synthase
25	T223_00515	119245..120192 [+], 948	568151284	hypothetical protein
26	T223_00520	120203..121117 [+], 915	568151285	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files

Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PA0085 , PA0091, PA0095, PA3484, PA2774, PA2702, PA2685, PA2684, PA1844

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	T223_00440	102430..102918 [+], 489	568151271	hypothetical protein	PA0085
2	T223_00470	110047..111987 [+], 1941	568151277	type VI secretion protein ImpA	PA0091
3	T223_00495	114379..116604 [+], 2226	568151280	type VI secretion protein ImpA	PA0095
4	T223_07610	1616602..1617828 [-], 1227	568152669	hypothetical protein	PA3484
5	T223_11640	2428633..2429220 [+], 588	568153442	hypothetical protein	PA2774
6	T223_12230	2537304..2537780 [-], 477	568153549	hypothetical protein	PA2702
7	T223_12315	2556812..2558881 [+], 2070	568153566	type VI secretion protein ImpA	PA2685
8	T223_12320	2558931..2562884 [+], 3954	568153567	hypothetical protein	PA2684
9	T223_17785	3810976..3811440 [+], 465	568154627	hypothetical protein	PA1844

Download FASTA format files

Proteins        Genes

Homology Immunity protein list

PA0092, PA3485, PA2775, PA2703, Tsi5, PA1845

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	T223_00475	112102..112386 [-], 285	568151278	hypothetical protein	PA0092
2	T223_07605	1616162..1616605 [-], 444	568152668	hypothetical protein	PA3485
3	T223_11635	2428191..2428628 [+], 438	568153441	hypothetical protein	PA2775
4	T223_12225	2537061..2537294 [-], 234	568153548	hypothetical protein	PA2703
5	T223_12325	2562881..2563072 [+], 192	568153568	hypothetical protein	Tsi5
6	T223_17780	3810416..3810934 [+], 519	568154626	hypothetical protein	PA1845

Download FASTA format files

Proteins        Genes

N/A