SecReT6
SecReT6 ID904 experimental
StrainEscherichia coli strain TW-XM
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionKF678349
Location1..29754
CommentT6SS1-associated mutants presented diminished adherence and invasion of several host cells cultured in vitro, decreased pathogenicity in duck and mouse infection models in vivo, and decreased biofilm formation and bacterial competitive advantage.
Functionanti-eukaryotic; effector translocation
T6SS TypeType i; subtype i2
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number111121111
ComponentTssGTssJTssKTssM
Number1111
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1- (xmoA)1..501 [+], 501611963679XmoA  TssB
2- (xmoB)554..2098 [+], 1545611963680XmoB  TssC
3- (xmoC)2116..3453 [+], 1338611963681XmoC  TssK
4- (xmoD)3450..4115 [+], 666611963682XmoD  TssL
5- (xmoE)4167..5780 [+], 1614611963683XmoE  TagL
6- (xmoF)5838..6329 [+], 492611963684Hcp-like protein  TssD
7- (xmoG)6521..9166 [+], 2646611963685putative ATP-dependent Clp protease  TssH
8- (xmoH)9178..11658 [+], 2481611963686XmoH  TssI
9- (xmoI)11678..13447 [+], 1770611963687hypothetical protein  Tle4 (e)
10- (xmoJ)13422..14132 [+], 711611963688hypothetical protein  Tli4 (i)
11- (xmoK)14223..14516 [+], 294611963689hypothetical protein 
12- (xmoL)14905..15240 [+], 336611963690hypothetical protein  PAAR
13- (xmoM)15161..16378 [+], 1218611963691hypothetical protein 
14- (xmoN)16388..19741 [+], 3354611963692IcmF-like protein  TssM
15- (xmoO)19707..21344 [+], 1638611963693XmoO  TssA
16- (xmoP)21344..22927 [+], 1584611963694hypothetical protein  PAAR
17- (xmoQ)22927..23556 [+], 630611963695hypothetical protein 
18- (xmoR)23813..24256 [+], 444611963696hypothetical protein 
19- (xmoS)24626..26386 [+], 1761611963697XmoS  TssF
20- (xmoT)26350..27429 [+], 1080611963698XmoT  TssG
21- (xmoU)27410..27814 [+], 405611963699XmoU  TssJ
22- (xmoV)27950..28378 [+], 429611963700XmoV  TssE
23- (xmoW)28378..29754 [+], 1377611963701XmoW  TssA
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
XmoF (Hcp1) , XmoI (Tle4)

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1-(xmoF)5838..6329 [+], 492611963684Hcp-like protein (Hcp1)XmoF (Hcp1)
2-(xmoI)11678..13447 [+], 1770611963687hypothetical protein (Tle4)XmoI (Tle4)
Data from experimental literature

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Proteins        Genes

Homology Immunity protein list
XmoJ (Tli4)

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1-(xmoJ)13422..14132 [+], 711611963688hypothetical protein (Tli4)XmoJ (Tli4)
Data from experimental literature

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Proteins        Genes
The information on regulator interaction.

#homolog effector locus tagRegulator wayFunctionRegulate targetDescription
1CXG97_03740 (Fur)Transcription regulator-T6SS1 (TssB) promotorFur directly suppressed the expression of hcp1 clusters through binding directly on the promotor of T6SS1 in the iron replete condition..
(1) Ma J et al. (2014). Two functional type VI secretion systems in avian pathogenic Escherichia coli are involved in different pathogenic pathways. Infect Immun. 82(9):3867-79. [PudMed:24980972] experimental
 
experimental This literature contains experimental investigation