I. Summary

SecReT6 ID	92
Strain	Burkholderia pseudomallei 1710b
Replicon	chromosome II [Browse all T6SS(s) in this replicon]
Accession	NC_007435
Location	715106..745670
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BURPS1710b_A0523	710622..710798 [-], 177	76817884	hypothetical protein
2	BURPS1710b_A0524	710900..711925 [+], 1026	76817355	N-acetylmuramoyl-L-alanine amidase
3	BURPS1710b_A0525	711922..712035 [-], 114	76817398	hypothetical protein
4	BURPS1710b_A0526 (tibC)	712053..713453 [+], 1401	76819374	hypothetical protein
5	BURPS1710b_A0527	712227..713705 [-], 1479	76819735	hypothetical protein
6	BURPS1710b_A0528	713890..715026 [+], 1137	76818786	Hep_Hag family protein
7	BURPS1710b_A0529	715106..715483 [+], 378	76818668	hypothetical protein
8	BURPS1710b_A0530	715726..717609 [-], 1884	76818412	ImpA-like N-terminal family protein	TssA
9	BURPS1710b_A0531 (virG)	717606..718343 [-], 738	76817810	DNA-binding response regulator	VirG
10	BURPS1710b_A0532	718340..720169 [-], 1830	76819512	sensor histidine kinase	VirA
11	BURPS1710b_A0534	720164..722446 [+], 2283	76817580	hypothetical protein	TssC
12	BURPS1710b_A0533	720429..720923 [+], 495	76818313	hypothetical protein	TssB
13	BURPS1710b_A0535	722666..723175 [+], 510	76819388	hypothetical protein	TssD
14	BURPS1710b_A0536	723102..723653 [-], 552	76817532	hypothetical protein
15	BURPS1710b_A0537	723168..723629 [+], 462	76817808	hypothetical protein	TssE
16	BURPS1710b_A0538	723666..725408 [+], 1743	76818402	hypothetical protein	TssF
17	BURPS1710b_A0539	725396..726418 [+], 1023	76818774	hypothetical protein	TssG
18	BURPS1710b_A0540	726405..729533 [+], 3129	76819348	ATP-dependent Clp protease, ATP-binding subunit ClpB	TssH
19	BURPS1710b_A0541	729560..732583 [+], 3024	76819027	Rhs element Vgr protein	TssI
20	BURPS1710b_A0542	732609..735251 [+], 2643	76819579	hypothetical protein
21	BURPS1710b_A0543	735269..736333 [+], 1065	76817838	hypothetical protein
22	BURPS1710b_A0544	736312..737082 [+], 771	76818346	hypothetical protein
23	BURPS1710b_A0545	737140..739740 [+], 2601	76817929	hypothetical protein	TssK
24	BURPS1710b_A0546	739737..740402 [+], 666	76819179	hypothetical protein	TssL
25	BURPS1710b_A0549	740509..744408 [+], 3900	76818261	hypothetical protein	TssM
26	BURPS1710b_A0547	742130..742243 [+], 114	76819403	hypothetical protein
27	BURPS1710b_A0548	742256..742453 [+], 198	76818689	hypothetical protein
28	BURPS1710b_A0550	744424..746364 [+], 1941	76819789	hypothetical protein	BMAA0729 (e)
29	BURPS1710b_A0551	745242..745670 [-], 429	76817554	hypothetical protein
30	BURPS1710b_A0552 (folE)	746775..747458 [+], 684	76819007	GTP cyclohydrolase I
31	BURPS1710b_A0553	747572..747895 [+], 324	76819810	putative transposase
32	BURPS1710b_A0554	748519..750048 [-], 1530	76819070	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files

Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

BMAA0737 , BMAA0742, BMAA0729

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	BURPS1710b_A0541	729560..732583 [+], 3024	76819027	Rhs element Vgr protein	BMAA0737
2	BURPS1710b_A0535	722666..723175 [+], 510	76819388	hypothetical protein	BMAA0742
3	BURPS1710b_A0550	744424..746364 [+], 1941	76819789	hypothetical protein	BMAA0729

Download FASTA format files

Proteins        Genes

N/A

(1) Boyer F et al. (2009). Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 10:104. [PudMed:19284603]

This literature contains bioinformatics investigation