I. Summary
Component | VgrG | ClpV | TssB | TssC | TssE | TssF | TssG | TssK | TssN |
Number | 2 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 |
Component | TssO | TssP | Hcp |
Number | 1 | 1 | 1 |
 |
III. genome coordinates of the T6SS gene cluster |
# | Locus tag (Gene) | Coordinates [+/-], size (bp) | Protein GI | Product | Note |
1 | PIN17_0479 | 512484..513977 [-], 1494 | 387132023 | RNA methylase, PF01170 family | |
2 | PIN17_0480 | 514325..515197 [-], 873 | 387132024 | sigma-70, region 3 | |
3 | PIN17_0481 | 515495..515650 [+], 156 | 387132025 | hypothetical protein | |
4 | PIN17_0483 | 517186..517644 [-], 459 | 387132026 | hypothetical protein | TssB |
5 | PIN17_0484 | 518018..518377 [-], 360 | 387132027 | hypothetical protein | |
6 | PIN17_0485 | 518470..518790 [-], 321 | 387132028 | hypothetical protein | |
7 | PIN17_0486 | 518787..519566 [-], 780 | 387132029 | PF08929 domain protein | |
8 | PIN17_0487 | 520222..520833 [-], 612 | 387132030 | PF08906 domain protein | |
9 | PIN17_0488 | 520847..522817 [-], 1971 | 387132031 | hypothetical protein | |
10 | PIN17_0489 | 522951..523940 [-], 990 | 387132032 | hypothetical protein | |
11 | PIN17_0490 | 523948..525057 [-], 1110 | 387132033 | phage-like baseplate assembly protein | VgrG |
12 | PIN17_0491 | 525013..525810 [+], 798 | 387132034 | hypothetical protein | |
13 | PIN17_0492 | 525887..526000 [+], 114 | 387132035 | hypothetical protein | |
14 | PIN17_0493 | 526012..526389 [-], 378 | 387132036 | hypothetical protein | Hcp |
15 | PIN17_0494 | 526973..527095 [+], 123 | 387132037 | hypothetical protein | |
16 | PIN17_0495 | 527118..527447 [+], 330 | 387132038 | putative lysozyme | TssE |
17 | PIN17_0496 | 527460..529217 [+], 1758 | 387132039 | hypothetical protein | TssF |
18 | PIN17_0497 | 529214..531655 [+], 2442 | 387132040 | hypothetical protein | ClpV |
19 | PIN17_0498 | 531640..532575 [+], 936 | 387132041 | hypothetical protein | TssG |
20 | PIN17_0499 | 532585..533469 [+], 885 | 387132042 | hypothetical protein | TssN |
21 | PIN17_0500 | 533474..534607 [+], 1134 | 387132043 | hypothetical protein | TssK |
22 | PIN17_0501 | 534622..535107 [+], 486 | 387132044 | hypothetical protein | TssO |
23 | PIN17_0502 | 535162..535566 [+], 405 | 387132045 | hypothetical protein | |
24 | PIN17_0503 | 535575..536549 [+], 975 | 387132046 | PKD domain-containing protein | TssP |
25 | PIN17_0504 | 536562..538829 [+], 2268 | 387132047 | hypothetical protein | |
26 | PIN17_0505 | 539110..539271 [+], 162 | 387132048 | hypothetical protein | |
27 | PIN17_0508 | 539910..540023 [-], 114 | 387132049 | hypothetical protein | |
28 | PIN17_0509 | 540100..540813 [-], 714 | 387132050 | hypothetical protein | |
29 | PIN17_0510 | 540778..541941 [+], 1164 | 387132051 | phage-like baseplate assembly protein | VgrG |
30 | PIN17_0511 | 541953..542801 [+], 849 | 387132052 | hypothetical protein | |
31 | PIN17_0512 | 542791..543945 [+], 1155 | 387132053 | hypothetical protein | |
32 | PIN17_0513 | 543966..544874 [+], 909 | 387132054 | hypothetical protein | |
|

Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein
V. Investigation of the genomic context of the T6SS gene cluster.