• T6SS
• Effector
• Immunity
• Adaptor
• Regulator
• Reference

SecReT6 ID	929
Strain	Serratia marcescens Db10
Replicon	T6SS [Browse all T6SS(s) in this replicon]
Accession	HG326223
Location	2365124..2402670
Comment	Killing of E. coli, P. fluorescens, Enterobacter cloacae, another strain of S. marcescens and susceptible mutants of Db10
Function	anti-bacterial
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	2	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

SMDB11_0927(Slp) , SMDB11_1083 (Tfe2), SMDB11_1112 (Ssp3), SMDB11_1610(Rhs2), SMDB11_1610 (Rhs2), SMDB11_2244 (VgrG), SMDB11_2261 (ssp1), SMDB11_2261 (Ssp1), SMDB11_2263 (Hcp), SMDB11_2264 (ssp2), SMDB11_2276 (VgrG), SMDB11_2278(Rhs1), SMDB11_2278 (Rhs1), SMDB11_3455 (Hcp), SMDB11_3456 (Hcp), SMDB11_3980 (Ssp4), SMDB11_4628 (Ssp5), SMDB11_4673 (Ssp6)   SMA3456 (Hcp protein)   SMA3455 (Hcp protein)   SMA4673 (Ssp6)   SMA4628 (Ssp5)   SMA3980 (Ssp4)   SMA2276 (VgrG)   SMA1112 (Ssp3)   SMA2244 (VgrG)   SMA2261 (Ssp1)   SMA2264 (Ssp2)   SMA2263 (Hcp protein)

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog

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Proteins        Genes

Homology Immunity protein list

SMDB11_0927 (Slp), SMDB11_0927 (Slp), SMDB11_1610 (Rhs2), SMDB11_2278 (Rhs1)   SMA2260 (Rap1a)   SMA2266 (Rap2b)   SMA4630 (Sip5b)   SMA2262 (Rap1b)   SMA2265 (Rap2a)   SMA4629 (Sip5a)   SMA3979 (Sip4)   SMA1111 (Sip3)

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog

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Proteins        Genes

Adaptors list

SMDB11_1609 (EagR2), SMDB11_2245 (EagR1)

Adaptor identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog

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Proteins        Genes

The information on regulator interaction.

#	homolog effector locus tag	Regulator way	Function	Regulate target	Description
1	SMDB11_2275 (PpKA)	TPP	+	SMDB11_2267 (FHA)	Phosphorylation of Fha by PpkA promotes formation ofFha foci and assembly ofan active T6SS in Serratiamarcescens.
2	SMDB11_2268 (PppA)	TPP	-	SMDB11_2267 (FHA)	PppA-mediated dephosphorylation of Fha is required for partial disassembly and re-assembly of the T6SS at a new location in the cell.
3	SMDB11_2269 (RtkS)	TPP	+	SMDB11_2275 (PpKA)	RtkS is an upstream activator of PpkA which stabilises the kinase through a direct interaction.
4	SMDB11_2256 (TagF)	TPP	-	unknown	TagF negatively regulates assembly ofa functional T6SS and that PpkA-mediated phosphorylation is required to overcome this repression.

(1) Srikannathasan V et al. (2013). Structural basis for type VI secreted peptidoglycan DL-endopeptidase function, specificity and neutralization in Serratia marcescens. Acta Crystallogr D Biol Crystallogr. 69(Pt 12):2468-82. [PudMed:24311588]

(2) Heymann JB et al. (2013). Three-dimensional structure of the toxin-delivery particle antifeeding prophage of Serratia entomophila. J Biol Chem. 288(35):25276-84. [PudMed:23857636]

(3) Fritsch MJ et al. (2013). Proteomic identification of novel secreted anti-bacterial toxins of the Serratia marcescens Type VI secretion system. Mol Cell Proteomics. 12(10):2735-2749. [PudMed:23842002]

(4) English G et al. (2012). New secreted toxins and immunity proteins encoded within the Type VI secretion system gene cluster of Serratia marcescens. Mol Microbiol. 86(4):921-36. [PudMed:22957938]

(5) Rao VA et al. (2011). The structure of Serratia marcescens Lip, a membrane-bound component of the type VI secretion system. Acta Crystallogr D Biol Crystallogr. 67(Pt 12):1065-72. [PudMed:22120744]

(6) Murdoch SL et al. (2011). The opportunistic pathogen Serratia marcescens utilizes type VI secretion to target bacterial competitors. J Bacteriol. 193(21):6057-69. [PudMed:21890705]

This literature contains experimental investigation

This literature contains bioinformatics investigation

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