SecReT6
SecReT6 ID929 experimental
StrainSerratia marcescens Db10
RepliconT6SS [Browse all T6SS(s) in this replicon]
AccessionHG326223
Location2365124..2402670
CommentKilling of E. coli, P. fluorescens, Enterobacter cloacae, another strain of S. marcescens and susceptible mutants of Db10
Functionanti-bacterial
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number111211111
ComponentTssGTssJTssKTssM
Number1111
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
 

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
SMDB11_0927(Slp) , SMDB11_1083 (Tfe2), SMDB11_1112 (Ssp3), SMDB11_1610(Rhs2), SMDB11_1610 (Rhs2), SMDB11_2244 (VgrG), SMDB11_2261 (ssp1), SMDB11_2261 (Ssp1), SMDB11_2263 (Hcp), SMDB11_2264 (ssp2), SMDB11_2276 (VgrG), SMDB11_2278(Rhs1), SMDB11_2278 (Rhs1), SMDB11_3455 (Hcp), SMDB11_3456 (Hcp), SMDB11_3980 (Ssp4), SMDB11_4628 (Ssp5), SMDB11_4673 (Ssp6)   SMA3456 (Hcp protein)   SMA3455 (Hcp protein)   SMA4673 (Ssp6)   SMA4628 (Ssp5)   SMA3980 (Ssp4)   SMA2276 (VgrG)   SMA1112 (Ssp3)   SMA2244 (VgrG)   SMA2261 (Ssp1)   SMA2264 (Ssp2)   SMA2263 (Hcp protein)

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

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Proteins        Genes

Homology Immunity protein list
SMDB11_0927 (Slp), SMDB11_0927 (Slp), SMDB11_1610 (Rhs2), SMDB11_2278 (Rhs1)   SMA2260 (Rap1a)   SMA2266 (Rap2b)   SMA4630 (Sip5b)   SMA2262 (Rap1b)   SMA2265 (Rap2a)   SMA4629 (Sip5a)   SMA3979 (Sip4)   SMA1111 (Sip3)

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

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Proteins        Genes

Adaptors list
SMDB11_1609 (EagR2), SMDB11_2245 (EagR1)

Adaptor identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

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Proteins        Genes
The information on regulator interaction.

#homolog effector locus tagRegulator wayFunctionRegulate targetDescription
1SMDB11_2275 (PpKA)TPP+SMDB11_2267 (FHA)Phosphorylation of Fha by PpkA promotes formation ofFha foci and assembly ofan active T6SS in Serratiamarcescens.
2SMDB11_2268 (PppA)TPP-SMDB11_2267 (FHA)PppA-mediated dephosphorylation of Fha is required for partial disassembly and re-assembly of the T6SS at a new location in the cell.
3SMDB11_2269 (RtkS)TPP+SMDB11_2275 (PpKA)RtkS is an upstream activator of PpkA which stabilises the kinase through a direct interaction.
4SMDB11_2256 (TagF)TPP-unknownTagF negatively regulates assembly ofa functional T6SS and that PpkA-mediated phosphorylation is required to overcome this repression.
(1) Srikannathasan V et al. (2013). Structural basis for type VI secreted peptidoglycan DL-endopeptidase function, specificity and neutralization in Serratia marcescens. Acta Crystallogr D Biol Crystallogr. 69(Pt 12):2468-82. [PudMed:24311588] experimental in_silico
(2) Heymann JB et al. (2013). Three-dimensional structure of the toxin-delivery particle antifeeding prophage of Serratia entomophila. J Biol Chem. 288(35):25276-84. [PudMed:23857636]
(3) Fritsch MJ et al. (2013). Proteomic identification of novel secreted anti-bacterial toxins of the Serratia marcescens Type VI secretion system. Mol Cell Proteomics. 12(10):2735-2749. [PudMed:23842002] experimental
(4) English G et al. (2012). New secreted toxins and immunity proteins encoded within the Type VI secretion system gene cluster of Serratia marcescens. Mol Microbiol. 86(4):921-36. [PudMed:22957938] experimental
(5) Rao VA et al. (2011). The structure of Serratia marcescens Lip, a membrane-bound component of the type VI secretion system. Acta Crystallogr D Biol Crystallogr. 67(Pt 12):1065-72. [PudMed:22120744]
(6) Murdoch SL et al. (2011). The opportunistic pathogen Serratia marcescens utilizes type VI secretion to target bacterial competitors. J Bacteriol. 193(21):6057-69. [PudMed:21890705] experimental
 
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation