I. Summary

SecReT6 ID	948
Strain	Aeromonas hydrophila NJ-35
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	CP006870
Location	2935924..2976020
Comment
Function	T6SS can secrete hcp and contributes to antiphagocytic activity against T.thermophila as well as the antibacterial property.
T6SS Type	Type i; subtype i1
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	2	1	1	2	2	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	U876_13425	2931295..2932245 [+], 951	827372781	thioredoxin reductase
2	U876_13430	2932303..2933550 [+], 1248	827372782	diguanylate cyclase
3	U876_13435	2933661..2934131 [-], 471	827372783	membrane protein
4	U876_13440	2934151..2934858 [+], 708	827372784	leucyl/phenylalanyl-tRNA--protein transferase
5	U876_13445	2934855..2935571 [+], 717	827372785	arginyl-tRNA-protein transferase
6	U876_13450	2935641..2935859 [+], 219	827372786	translation initiation factor IF-1
7	U876_13455 (clpA)	2935924..2938176 [-], 2253	827372787	Clp protease ClpX	TssH
8	U876_13460 (clpS)	2938236..2938553 [-], 318	827372788	Clp protease ClpS
9	U876_13465	2938782..2939000 [+], 219	827372789	stationary phase/starvation inducible regulatory protein CspD
10	U876_13470	2939107..2939970 [-], 864	827372790	methenyltetrahydrofolate cyclohydrolase
11	U876_13490	2940694..2940843 [+], 150	827372791	transposase
12	U876_13495	2942069..2944681 [-], 2613	827372792	hypothetical protein
13	U876_13500	2944800..2946848 [-], 2049	827372793	type IV secretion protein Rhs	TssI
14	U876_13505	2946859..2947146 [-], 288	827372794	hypothetical protein
15	U876_13510	2947404..2948834 [-], 1431	827372795	membrane protein	TssA
16	U876_13515	2948881..2952366 [-], 3486	827372796	type VI secretion protein IcmF	TssM
17	U876_13520	2952408..2953853 [-], 1446	827372797	type VI secretion protein	TssA
18	U876_13525	2953862..2954467 [-], 606	827372798	type VI secretion protein
19	U876_13530	2954467..2956005 [-], 1539	827372799	ATPase AAA
20	U876_13535	2956008..2958650 [-], 2643	827372800	Clp protease ClpV	TssH
21	U876_13540	2958671..2959450 [-], 780	827372801	type IV secretion protein DotU	TssL
22	U876_13545	2959473..2960807 [-], 1335	827372802	hypothetical protein	TssK
23	U876_13550	2960810..2961325 [-], 516	827372803	hypothetical protein	TssJ
24	U876_13555	2961325..2962575 [-], 1251	827372804	type VI secretion protein
25	U876_13560	2962632..2963630 [-], 999	827372805	type VI secretion protein	TssG
26	U876_13565	2963594..2965360 [-], 1767	827372806	type VI secretion protein	TssF
27	U876_13570	2965364..2965795 [-], 432	827372807	lysozyme	TssE
28	U876_13575	2965802..2967280 [-], 1479	827372808	type VI secretion protein	TssC
29	U876_13580	2967319..2967822 [-], 504	827372809	type VI secretion protein	TssB
30	U876_13590	2970004..2970549 [-], 546	827372810	hypothetical protein
31	U876_13595	2970673..2971662 [-], 990	827372811	hypothetical protein
32	U876_13600	2971672..2972991 [-], 1320	827372812	hypothetical protein
33	U876_13605	2972993..2975224 [-], 2232	827372813	type IV secretion protein Rhs	TssI
34	U876_13610	2975502..2976020 [-], 519	827372814	hypothetical protein	TssD
35	U876_13625	2977178..2977639 [-], 462	827372815	histidine kinase
36	U876_13630	2977792..2979129 [+], 1338	827372816	hypothetical protein
37	U876_13635	2979198..2979779 [-], 582	827372817	thymidine kinase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

U876_17565 (Hcp2) , U876_17750 (hcp1)

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	U876_17565	3836933..3837451 [-], 519	827373572	hypothetical protein (Hcp2)	U876_17565 (Hcp2)
2	U876_17750	3869323..3869841 [-], 519	827373605	hypothetical protein (hcp1)	U876_17750 (hcp1)
Data from experimental literature

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Proteins        Genes

Homology immunity protein (immunity protein label, when available)

(1) Nannan Wang, Jin Liu, Maoda Pang, Yafeng Wu, Furqan Awan, Mark R Liles, Chengping Lu, Yongjie Liu (2018). Diverse Roles of Hcp Family Proteins in the Environmental Fitness and Pathogenicity of Aeromonas Hydrophila Chinese Epidemic Strain NJ-35. Appl Microbiol Biotechnol. 102(16):7083-7095. [PudMed:29862449]

(2) Wang N et al. (2015). Protective efficacy of recombinant hemolysin co-regulated protein (Hcp) of Aeromonas hydrophila in common carp (Cyprinus carpio). Fish Shellfish Immunol. 46(2):297-304. [PudMed:26093203]

This literature contains experimental investigation