I. Summary
SecReT6 ID948 experimental
StrainAeromonas hydrophila NJ-35
Repliconchromosome [Browse all T6SS(s) in this replicon]
FunctionT6SS can secrete hcp and contributes to antiphagocytic activity against T.thermophila as well as the antibacterial property.
T6SS TypeType i; subtype i1
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1U876_134252931295..2932245 [+], 951827372781thioredoxin reductase 
2U876_134302932303..2933550 [+], 1248827372782diguanylate cyclase 
3U876_134352933661..2934131 [-], 471827372783membrane protein 
4U876_134402934151..2934858 [+], 708827372784leucyl/phenylalanyl-tRNA--protein transferase 
5U876_134452934855..2935571 [+], 717827372785arginyl-tRNA-protein transferase 
6U876_134502935641..2935859 [+], 219827372786translation initiation factor IF-1 
7U876_13455 (clpA)2935924..2938176 [-], 2253827372787Clp protease ClpX  TssH
8U876_13460 (clpS)2938236..2938553 [-], 318827372788Clp protease ClpS 
9U876_134652938782..2939000 [+], 219827372789stationary phase/starvation inducible regulatory protein CspD 
10U876_134702939107..2939970 [-], 864827372790methenyltetrahydrofolate cyclohydrolase 
11U876_134902940694..2940843 [+], 150827372791transposase 
12U876_134952942069..2944681 [-], 2613827372792hypothetical protein 
13U876_135002944800..2946848 [-], 2049827372793type IV secretion protein Rhs  TssI
14U876_135052946859..2947146 [-], 288827372794hypothetical protein 
15U876_135102947404..2948834 [-], 1431827372795membrane protein  TssA
16U876_135152948881..2952366 [-], 3486827372796type VI secretion protein IcmF  TssM
17U876_135202952408..2953853 [-], 1446827372797type VI secretion protein  TssA
18U876_135252953862..2954467 [-], 606827372798type VI secretion protein 
19U876_135302954467..2956005 [-], 1539827372799ATPase AAA 
20U876_135352956008..2958650 [-], 2643827372800Clp protease ClpV  TssH
21U876_135402958671..2959450 [-], 780827372801type IV secretion protein DotU  TssL
22U876_135452959473..2960807 [-], 1335827372802hypothetical protein  TssK
23U876_135502960810..2961325 [-], 516827372803hypothetical protein  TssJ
24U876_135552961325..2962575 [-], 1251827372804type VI secretion protein 
25U876_135602962632..2963630 [-], 999827372805type VI secretion protein  TssG
26U876_135652963594..2965360 [-], 1767827372806type VI secretion protein  TssF
27U876_135702965364..2965795 [-], 432827372807lysozyme  TssE
28U876_135752965802..2967280 [-], 1479827372808type VI secretion protein  TssC
29U876_135802967319..2967822 [-], 504827372809type VI secretion protein  TssB
30U876_135902970004..2970549 [-], 546827372810hypothetical protein 
31U876_135952970673..2971662 [-], 990827372811hypothetical protein 
32U876_136002971672..2972991 [-], 1320827372812hypothetical protein 
33U876_136052972993..2975224 [-], 2232827372813type IV secretion protein Rhs  TssI
34U876_136102975502..2976020 [-], 519827372814hypothetical protein  TssD
35U876_136252977178..2977639 [-], 462827372815histidine kinase 
36U876_136302977792..2979129 [+], 1338827372816hypothetical protein 
37U876_136352979198..2979779 [-], 582827372817thymidine kinase 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
U876_17565 (Hcp2) , U876_17750 (hcp1)

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1U876_175653836933..3837451 [-], 519827373572hypothetical protein (Hcp2)U876_17565 (Hcp2)
2U876_177503869323..3869841 [-], 519827373605hypothetical protein (hcp1)U876_17750 (hcp1)
Data from experimental literature

Download FASTA format files
Proteins        Genes
Homology immunity protein (immunity protein label, when available)
(1) Nannan Wang, Jin Liu, Maoda Pang, Yafeng Wu, Furqan Awan, Mark R Liles, Chengping Lu, Yongjie Liu (2018). Diverse Roles of Hcp Family Proteins in the Environmental Fitness and Pathogenicity of Aeromonas Hydrophila Chinese Epidemic Strain NJ-35. Appl Microbiol Biotechnol. 102(16):7083-7095. [PudMed:29862449] experimental
(2) Wang N et al. (2015). Protective efficacy of recombinant hemolysin co-regulated protein (Hcp) of Aeromonas hydrophila in common carp (Cyprinus carpio). Fish Shellfish Immunol. 46(2):297-304. [PudMed:26093203] experimental
experimental This literature contains experimental investigation